Relationship Between GRP78,CHOP And Stability Of Carotid Atherosclerotic Plaque In Unfolded Protein Response

Objective: Ischemic stroke is the main killer of national health and is currently growing at a rate of 9% per year.The pathophysiological changes such as the formation,rupture and hemorrhage of human carotid atherosclerotic plaque are important causes of ischemic stroke.The current research hotspots are mainly aimed at unstable plaques.To investigate the expression of endoplasmic reticulum stress(ERS)-related factors GRP78 and CHOP in different types of human carotid intima,and to explore the role of endoplasmic reticulum stress in the development and progression of carotid intima plaque formation.To find new diagnostic biomarkers for the diagnosis of unstable plaques,providing a new entry point for clinical prevention and treatment of atherosclerosis.Methods:A total of 30 cases of endarterial plaque specimens after carotid endarterectomy(CEA)at the Dalian Central Hospital from January 2017 to December2018 were selected.According to pathological classification,it can be divided into two groups: stable plaque and Vulnerable plaque.The specimens were immunohistochemically,and the GRP78 and CHOP proteins in the endometrial cells were qualitatively and localized.Western blot and RT-qPCR were used to quantitatively measure the expression levels of GRP78 and CHOP in the specimens.The obtained data were statistically analyzed by SPSS 21.0 statistical software.The data of each group of experimental data were expressed as mean±standard deviation(mean±SD),and the difference between groups was analyzed by independent sample t test.The correlation between the two was used.The Levene variance homogeneity test,P < 0.05,considered the difference to be statistically significant.The statistical mapping software used Graph pad Prism 6.0.Chi-square test was used to explain the correlation between the clinical relationship of patients and the expression of GRP78 and CHOP molecules in endoplasmic reticulum stress.Results:1.Immunohistochemistry,Western blot and RT-qPCR were used to detect the expression of GRP78 and CHOP in different plaques,indicating differential expression(P<0.05).The expression of endoplasmic reticulum stress factors GRP78 and CHOP in unstable plaques was higher than that of stable plaques.2.GRP78 and CHOP have a certain diagnostic value as a marker molecule for diagnosing unstable plaques.The CHOP molecule(AUC=0.813)has more diagnostic value than GRP78(AUC=0.778)..3.By analyzing the expression of GRP78 m RNA in carotid intima tissue,the high expression of GRP78 was only related to gender,and statistical analysis showed statistical significance(?2 =4.658 P= 0.031<0.05).By statistical analysis of patients' age,ABCD-2 score,low-density lipoprotein,location,whether combined with hypertension,diabetes,and whether smoking,several factors were used to analyze the difference in the expression of GRP78 in these factors,and found no statistics.significance.Conclusions: 1.GRP78 and CHOP were differentially expressed in human carotid endometrial plaques of different pathological types(P<0.05).The expression of GRP78 and CHOP in pathological types was significantly higher than that of stable plaques..This indicates that the protein plays a role in the occurrence and development of As.2.GRP78 and CHOP have diagnostic significance as markers for diagnosing unstable plaques.Later studies require repeated verification and continue to search for more diagnostic markers.3.The expression of GRP78 and CHOP protein in carotid intima plaque has certain correlation with various factors in clinical patients.However,in view of the small sample size obtained in this study,whether the correlation is statistically significant remains to be further studied.