Role Of ?-1,3-Galactosyltransferase 2 Gene Knockout In Hippocampal Neuroinflammation Of Aged Mice

ObjectiveGlycosyltransferase is one of the most important enzymes in the neurodevelopment.?-1,3-galactosyltransferase 2(B3galt2)belongs to ?-1,3-galactosyltransferase,which is one of the major types of glycosyltransferases.This study explores the role of B3galt2 in the hippocampal neuroinflammation induced by aging,and its mechanism.It provides a theoretical basis for the further study of the role of B3galt2 in the central nervous system.MethodB3galt2 knockout heterozygote mice were used for breeding wild type,heterozygote and homozygote mice.The changes of microglia in hippocampus were observed by immunohistochemical staining.The changes of IL-1? and TNF-? in hippocampus were observed by Western blotting.The expression of ?-amyloid plaques and MAP2 in hippocampus of aging mice was observed by immunofluorescence staining.Results1.Wild type,heterozygote and homozygote mice from the same litter and sex were successfully obtained by breeding B3galt2 heterozygotes.2.B3galt2 gene knockout reduced the expression of IL-1? and TNF-?.3.B3galt2 gene knockout lead to a decrease in the number of microglia.4.B3galt2 knockout increased the deposition of ?-amyloid plaques in hippocampal region of aging mice.5.B3galt2 knockout reduced the dendrites of neurons that labeled by MAP2 in hippocampal region of aging mice.Conclusion1.In aging mice,B3galt2 knockout reduce neuroinflammation in the hippocampus.2.B3galt2 gene knockout could reduce the activation of microglia and the expression of MAP2,increase the deposition of A? amyloid protein.3.The knockdown of B3galt2 gene may attenuate the protective effect on neurons.