BRCA1,NEK2,GPSM2,MDK Novel Networks Construction And Analysis In HCC Compared With Hepatitis/cirrhotic Liver Tissues Based On Computation

BRCA1,NEK2,GPSM2,MDK molecular and knowledge networks were originally reconstructed,put forward and verified the novel hypothesis in human hepatocellular carcinoma(HCC)from GEO Dataset GSE10140-10141 by gene selection algorithm significance analysis of microarrays(SAM),gene network reconstruction infer(GNRInfer),the database for annotation visualization and integrated discovery(DAVID),molecule annotation system(MAS).BRCA1 different cell cycle activated network was computed in HCC compared with no-tumor hepatitis/cirrhotic liver tissues.Our computational results shows cell cycle,negative regulation of apoptotic process,negative regulation of cysteine type endopeptidase activity involved in apoptotic process,negative regulation of apoptosis,negative regulation of programmed cell death,negative regulation of ubiquitin protein ligase activity involved in mitotic cell cycle,etc.Therefore,we put forward that BRCA1 activated apoptosis negative regulation-related cell cycle network in HCC,and they are verified mutually.NEK2 different cell cycle activated network was computed in HCC compared with no-tumor hepatitis/cir-rhotic liver tissues.Our computational results illustrates regulation of cell cycle,mitotic cell cycle,transcription from RNA polymerase ? promoter,transcription factor activity sequence specific DNA binding,positive regulation of transcription DNA templated,positive regulation of transcription from RNA polymerase ? promoter,transcription factor binding,transcription cofactor activity,etc.Therefore,we put forward that NEK2 activated transcription-related cell cycle regulation network in HCC,and they are verified mutually.GPSM2 different signal transduction activated network was computed in HCC compared with no-tumor hepatitis/cirrhotic liver tissues.Our computational results reveals the signaling pathway of cell cell signaling,MAPK,small GTPase,cell surface receptor,I kappaB kinase/NF kappaB,tumor necrosis factor,extrinsic apoptotic,RIG I like receptor,adipocytokine,p38 MAPK,tumor necrosis factor receptor binding,CD40 receptor binding,etc.Therefore,we put forward that GPSM2 activated cell cell signaling network in HCC,and they are verified mutually.MDK different signal transduction activated network was computed in HCC compared with no-tumor hepatitis/cirrhotic liver tissues.Our computational results demonstrates the signaling pathway of intracellular signal transduction,MAPK activity,Ras,adenylate cyclase modulating and inhibiting G protein coupled receptor,positive regulation of peptidyl serine phosphorylation,oxytocin,calmodulin dependent protein kinase,ATP binding,etc.Therefore,we put forward that MDK activated intracellular signal transductiong network in HCC,and they are verified mutually.