| Uric acid is the final oxidation product of the metabolism of human and other higher primates,and is one of the most important antioxidants in the body.Long-term high uric acid will cause deposition of urate in the joints and kidneys,which is the essential cause of gout.Gout patients often have tophus,which can lead to joint deformity,dysfunction,compression nerves,and skin ulceration.The kidneys are also damaged throughout the onset of the disease.In severe cases,kidney stones or even kidney failure can occur,which is life-threatening.Therefore,in order to prevent and diagnose gout early,it is urgent to develop a novel method for simple and rapid quantification of uric acid.In this study,a new method for quantitative analysis of uric acid in human saliva and nails was established by high performance liquid-ultraviolet(HPLC-UV)detector.We used Inertsil ODS-3(250 mm × 4.6 mm,4 ?m)column and the column temperature is 40?.Mobile phase A is methanol,mobile phase B is phosphate buffer.And their radio is 2:98.The flow rate was 1 min/mL,and the analysis was performed within 7 minutes under the wavelength of 284 nm to detect.The linear regression equation of uric acid was y=0.5333x-0.4128(R2 ? 0.9999),and a good linearity was achieved was in the range of 0.5-600 ?g/mL.The lostest detection limit(S/N=3)was 5 ng/mL.The precision ranged is from 0.54 to 6.42%.The best collection time for saliva is 8:30-11:30 and 16:30-18:30.Saliva at room temperature within 24 hours,three freeze-thaw cycle and long-term freeze-thaw cycle is still stable.The recovery rate of uric acid in saliva of healthy volunteers was 98.20-105.99%.The average recovery was 102.48%.The intraday precision was 0.24-2.56%and the intreday precision was 1.62-2.22%.The recovery rate of uric acid in saliva of patients with gout is 101.50-106.16%.The average recovery was 103.73%.The intraday precision was 0.89-3.94%.The intreday precision was 1.47-2.79%.The recovery rate of uric acid in the nails of healthy volunteers was 90.80-106.90%.The average recovery rate is 98.40%.The intraday precision is 1.44-5.87%,and the intreday precision is 1.09-7.99%.The average content of uric acid in saliva of healthy volunteers was 35.26±14.06 ?g/mL,and the average content of uric acid in saliva of patients with gout was 114.61±67.50 ?g/mL.The average content of uric acid in saliva of patients with gout was significantly higher than that of healthy people.There was a significant correlation between the average uric acid content in saliva of patients with gout and the average uric acid content in saliva of healthy people(***P<0.001).There was a statistical difference between them.The average content of uric acid in nails of healthy volunteers was 29.95±31.05 ng/mg,and the average content of uric acid in nails of gout patients was 156.63±185.09 ng/mg.The average content of uric acid in nails of gout patients was significantly higher than that in healthy people.there was a significant correlation between the average content of uric acid in nails of healthy volunteers and the average content of uric acid in nails of gout patients(***P<0.001).There was a statistical difference between them.The content of uric acid in the nails of healthy volunteers(ZJ)was relatively stable within 12 weeks.In patients with gout treated with medication(fbst),uric acid levels in the nails tended to decrease over a 12 week period with treatment.The content of uric acid in the fingernail has the same tendency as the content of uric acid in the plasma.A novel analytical method for quantitative analysis of uric acid in human saliva and nails was established.The model for early prevention,diagnosis and treatment of gout diseases would be conducted. |
PDF Full Text Request