Preliminary Study On Clinical Value Of CD138 In Premature Rupture Of Membranes

Research BackgroundPremature rupture of membrane(PROM)refers to the natural rupture of the membrane before labor,which is a common complication of perinatal period,which greatly increases the infection rate of pregnant women and fetuses and the mortality rate of perinatal children.The cause of PROM is generally thought to be related to various factors such as the structure of the membrane itself,genital tract infection,increased amniotic pressure,and uneven membrane force.Its occurrence is the result of the interaction of various factors,and the exact cause of its occurrence is unknown.However,the current unified view is that the defects of inflammation and the structure of the membrane itself are the basic pathological changes.CD138(syndecan-1,Sdc-1)is a member of the syndecan family of heparan sulfate proteoglycans(HSPGs),also known as multiligandin-1,which is expressed primarily in epithelial and plasma cells.CD138 is important for cell-cell and cell-matrix interactions.In infectious diseases,CD138 is an important adhesion molecule,and various microorganisms such as bacteria,viruses,and parasites adhere to host cells through CD138 as an adhesion receptor,and participate in certain inflammatory reactions.The exfoliation of CD138 may disturb the balance between pro-inflammatory and anti-inflammatory responses,increase the activation of inflammatory cells,and thus intensify the process of inflammation.In recent years,research on CD138 has focused on tumors,and no relevant reports have been reported in the field of obstetrics.Research ObjectiveIn this study,CD138 and premature rupture of membranes will be used to observe the expression of CD138 in each group of fetal membranes and the prognosis of neonates.The relationship between CD138 and premature rupture of membranes and neonates will be further analyzed.To study the mechanism of action of CD138 in premature rupture of membranes,and provide reference for clinical study to reduce the occurrence of adverse outcomes in mothers and children.Materials and Methods30 cases of premature rupture of membranes in the Department of Obstetrics and Gynecology,the Fifth Affiliated Hospital of Zhengzhou University from September 2017 to July 2018,and 30 cases of premature rupture of membranes were randomly selected according to the inclusion criteria.30 cases of pregnant women and 30 cases of normal delivery.The expression of CD138 in the three groups of fetal membranes was determined by immunohistochemistry.The expression of CD138 in the three groups was analyzed according to the experimental results and the relationship with the postpartum condition of the newborn.Research resultsThere were no significant differences in age,height and weight between the three groups(P>0.05).Immunohistochemistry results showed that CD138 expression was statistically different between the three groups(P<0.05).There were statistically significant differences between the three groups of neonatal birth and postpartum outcomes(P<0.05).Analysis of the relationship between neonatal and CD13 expression showed that there was a statistically significant difference in the incidence of pneumonia between the positive and negative groups of CD138 in the preterm premature rupture of membranes(P<0.05),the other no significant difference(P>0.05).ConclusionThere was a statistically significant difference in the expression of CD138 between the three groups,and there were statistical differences in the outcomes of the three groups of neonatal prognosis.We hypothesized that CD138 may be involved in the premature rupture of membranes through the mechanism of inflammatory response.We intend to explore the mechanism of action of CD138 in premature rupture of membranes in the next phase of research.