| Objectives:To investigate the role of long-chain non-coding RNA LIMT(LINC01089)in Hepatocellular carcinoma(HCC)and its relationship with transforming growth factor-?1(TGF-?1)signaling pathway and epithelial-mesenchymal transition(EMT).Methods:HCC tissues and corresponding non-tumor tissues were collected from 65 patients in Zhengzhou University People's Hospital who underwent liver cancerresection with complete case data.Real-time quantitative reverse transcriptase-polymerase chain reaction(qRT-PCR)was used to detect the expression of lncRNA LIMT in HCC tissues and corresponding adjacent tissues.The paired sample t-test was used to analyze the difference of lncRNA LIMT expression between the two groups.At the same time,the chi-square test was used to analyze the relationship between the expression of lncRNA LIMT and clinicopathological features in HCC tissues.The expression of lncRNA LIMT was analyzed by independent sample t-test.The expression of TGF-?1,Smad4,E-cadherin,N-cadherinin TGF-?1 pathway and EMT were detected by immunohistochemistry.Chi-square test and Spearman rank correlation analysis were used to analyze the correlation between lncRNA LIMT,TGF-?1 signaling pathway and EMT in HCC.The obtained data were statistically analyzed using SPSS 22.0 software and plotted using GraphPad prism 8.0 software.Results:1.The relative expression of lncRNA LIMT in HCC tissues was(1.08±0.36),which was significantly lower than that in corresponding adjacent tissues(1.96 ±0.48).The difference was statistically significant(P<0.01).2.The expression level of lncRNA LIMT in hepatocellular carcinoma tissues was significantly correlated with the degree of differentiation,portal vein thrombosis(P<0.05).There was no significant difference in gender,age,tumor size,alcohol abuse history,combined virus(HBV+HCV)infection,cirrhosis,AFP and TNM staging(P>0.05).In HCC tissues,the expression level of lncRNA LIMT in the poorly differentiated group was lower than that in the middle-high differentiation group.The expression of lncRNA LIMT in the portal vein tumor thrombus group was lower than that in the no portal vein tumor thrombus group(P<0.01).3.The positive expression rate of TGF-?1 in HCC tissues was 53.8%(35/65),and the positive expression rate in non-tumor tissues was 35.4%(23/65)(P<0.05).The positive expression rate of Smad4 in HCC tissues was 40%(26/65)lower than that in non-tumor tissues 63.1%(41/65)(P<0.01).4.The positive expression rate of E-cadherin in HCC tissues was 41.5%(27/65),which was lower than that in adjacent tissues(63.1%,41/65)(P<0.05).The positive expression rate of N-cadherin in HCC tissues was 67.7%(44/65),which was higher than that in adjacent tissues was 43.1%(28/65).The difference was statistically significant(P<0.01).5.There was a negative correlation between TGF-?1 and E-cadherin expression in HCC tissues,which was positively correlated with N-cadherin expression.There was a positive correlation between Smad4 and E-cadherin expression in HCC tissues,which was negatively correlated with N-cadherin expression.6.The expression of LncRNA LIMT was negatively correlated with the expression of TGF-?1 and N-cadherin in HCC tissues(P<0.05).The expression of LncRNA LIMT in HCC tissues was negatively correlated with the expression of Smad4 and E-cadherin(P<0.01).Conclusion:1.LncRNA LIMT showed low expression in HCC tissues compared with adjacent tissues.The patients who with lower degree of differentiation and portal vein thrombosis in hepatocellular carcinoma,the lower the expression of lncRNA LIMT,suggesting that lncRNA LIMT plays a role in the development of HCC..2.The expression of TGF-?1 and N-cadherin was up-regulated in HCC tissues,and the expression of Smad4 and E-cadherin was down-regulated.The occurrence and development of HCC may be closely related to the abnormality of TGF-?1signaling pathway and EMT process.3.In HCC,lncRNA LIMT is associated with TGF-?1 signaling pathway and EMT,which may affect EMT process through TGF-?1 signaling pathway. |
PDF Full Text Request