| Objective:1.The acute toxicity test of taohong siwu decoction was conducted to provide theoretical basis for the rational selection of clinical dosage forms and drug safety of taohong siwu decoction.2.Taking the open femoral shaft fracture model of rats as the research object,the intervention model group with different doses of taohong siwu decoction was used to observe the effects of different doses on the fracture healing and serum VEGF of the open femoral shaft fracture model of rats,and to explore the dose-effect relationship of taohong siwu decoction.Methods:1.40 KM mice(SPF level)were selected and randomly divided into the taohong siwu decoction group and the blank control group.In the taohong siwu decoction group,the maximum dosage concentration and the maximum volume were given by gavage,while in the blank control group,the same volume of normal saline was given by gavage 3 times a day,with an interval of 6 hours.After 14 days of continuous gavage,the mice were observed for death,eating and drinking,abnormal activities,pupil changes,eyeball and eyelid changes,defecation and urine secretion,etc.After 14 days,mice were sacrificed for histopathological observation of heart,lung,liver,kidney and other organs.2.Seventy-two healthy SD male rats(SPF grade)were selected and randomly divided into blank group,sham operation group,model group and taohong siwu decoction low,medium and high dose groups,with 12 rats in each group.In addition to the blank group and the sham operation group,the remaining open femoral shaft fracture model was used to establish the rat model.After successful modeling,the dosage was calculated according to the conversion formula of human and animal surface area.The blank control group,the sham operation group and the model group were given normal saline every day,and the low,medium andhigh dose groups were given taohong siwu decoction every day.The whole process lasted for10 days.The general situation of the rats,the degree of limb swelling,and 4 rats were randomly selected from each group on the 4th,7th,and 10 th days after gavage,and the samples were sacrificed for microscopic CT and histopathological analysis.The difference of VEGF expression in serum was detected by Elisa.2.Results:1.There was no death of mice in the Taohong siwu decoction group and the blank control group,and no obvious abnormality was found in the general condition of mice and the pathological examination of main organs.The lethal dose(LD50)of Taohong siwu decoction was not determined,and the maximum dose of mice was 144 times of the daily oral dose of adults.2.After modeling,compared with the blank group and the sham operation group,the rats in the model group showed different degrees of poor spirit,reduced diet,red and swollen fracture ends,high local skin temperature,and increased limb swelling.Local anatomy showed good closure of fracture ends,and kirchner wire fixation,indicating successful modeling.Compared with the model group,the general condition and fracture healing of the rats in each dose group of traditional Chinese medicine were improved,the micro-ct and histopathology showed increased callus at the fracture end,and the expression of serum VEGF was significantly increased.There was a dose-effect relationship between the groups of taohong siwu decoction,showing a positive correlation,and the high-dose group was the best.Conclusion:1.Maximum gavage was used in the acute toxicity test of taohong siwu decoction,and it was found that there was no adverse reaction in mice and no obvious damage to important organs in the test,indicating that taohong siwu decoction was safe to be taken at a conventional dose.2.Taohong siwu decoction significantly promoted the fracture healing and VEGF expression in rats;The curative effect was positively correlated with dose,and the high dose group had the best curative effect. |
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