Immunogenicity Of Multiple Antigenic Peptide System Coupled With Amyloid Beta Protein Epitopes

Latest studies suggest that Alzheimer's disease?AD?is one of the"four big killers"that threaten the health of the elderly.AD affects about 46 million people across the world,and there is a critical need for research on new therapies for treating AD.The purpose of this study was to develop and evaluate immunization strategies to treat AD by inhibiting the formation of amyloid beta protein?A??,a pathological hallmark of AD.In this study,serial potential immunogenic epitopes were screened based on the A?_?1-42?sequence.Next,the screened linear epitope on the A?C-terminal fragment was coupled with either the carrier protein keyhole limpet hemocyanin?KLH?or the synthesized 4-branched peptide?MAP₄?.Further,Balb/c mice were immunized with both the vaccines.Our results suggest that MAP₄ could effectively induce A?antibody titers and the monoclonal antibody 1H7 specifically recognized A?monomer and fiber.Additionally,1H7 could inhibit the formation of the A?fiber and also depolymerize it.In conclusion,the low molecular weight of MAP₄ might give it a very broad application in preventing the inflammatory response and being a potential candidate for the development of a safer and more effective AD vaccine.