The Role Of STOML2 Protein In The Development Of Colorectal Cancer And Preliminary Study Of Its Interacting Protein

Research background and significanceColorectal cancer is a common and high incidence of malignant tumors all over the world.According to statistics,the total number of new cases of colorectal cancer in the United States in 2018 is about 140250,ranking fourth;the number of deaths is estimated to be about 50630,ranking third.With the development of the economy,the seriously aging trend of population,the increasing number of obesity or diabetes population,the increasing consumption of meat products,The incidence of colorectal cancer is expected to continue to rise.Colorectal cancer poses a heavy burden on patients,families and society,which is a serious public health and social problem.At present,the treatment of colorectal cancer is still a comprehensive treatment based on surgery.Although significant progress has been made,it is still not effective for locally advanced or metastatic colorectal cancer and with poor prognosis.With the development work on the epidemiology,etiology,clinical diagnosis and prevention of colorectal cancer,it is now widely accepted that colorectal cancer is a malignant tumor involving multiple gene mutations and step by step process.Colorectal cancer is closely related to heredity,environment,intestinal flora,chronic intestinal inflammation and so on.Gene mutation plays a key role in the development of colorectal cancer.The current molecular markers of colorectal cancer,such as EGFR and VEGF,have been used for clinical diagnosis and treatment,but the effect still is not satisfactory.In order to develop new targets for colorectal cancer,We selected the stomatin like protein 2(STOML2),which was first reported in 2000 by Wang,Y,working in the Department of Pathology,Yale University.It has been proved that the increase in the expression of STOML2 protein is associated with a variety of malignant tumors and suggests poor prognosis.The relationship between STOML2 protein and colorectal cancer has also been reported by some authors.For example,ELISA detection had showed that the blood STOML2 level increased in early CRC cases,with relatively high sensitivity.When combined with other tumor antigen testing,it would significantly.improve the sensitivity and specificity of colorectal cancer.But in general,there is insufficient research focused on the mechanism of STOML2 for the promotion of colorectal cancer.MethodIn the first stage,we identified a number of publicly published and accessible databases by searching online database resources,which contain huge amounts of information about gene chips and RNA sequencing.Through the extraction,statistical analysis and mapping of the data,we can find that the expression of STOML2 in colorectal cancer tissue increased significantly,and it was confirmed in multiple bioinformatics databases.For the next step,we collect the Paraffin specimen from our department in the hospital.Using immunohistochemical assay,it is confirmed that STOML2 has a significant increase in colorectal cancer comparing to adjacent normal tissue.There is a significant correlation between the level of the increase and the stage of the disease.The overexpression of STOML2 correlated with poor clinical outcome.Finally,to investigate which role STOML2 plays in colorectal cancer,we designed a yeast two hybrid experiment to detect proteins that interact with STOML2 in a colorectal cell line.Based on the results,we find proteins interacting with STOML2,and the next step research for STOML2 in colorectal cancer needs to be carried out.ConclusionsSTOML2 is overexpressed in colorectal cancer and is associated with poor prognosis.The proteins interacting with STOML2 were successfully screened by yeast two-hybrid experiment,which lays the foundation for the next step research.