Expression And Significance Of E-cadherin,EGFR And AKT,c-Myc,FOXO3a And ATP4b In Gastric Cancer

ObjectiveTo study the expression and significance of epithelial cadherin(E-cadherin),epidermal growth factor receptor(EGFR)and protein kinase B(AKT),c-Myc,FOXO3 a and ATP4 b in gastric cancer tissues and cells,and to analyze their correlation with clinicopathological parameters.The possible mechanism of gastric cancer was preliminarily discussed to provide basic theoretical reference for individualized treatment of gastric cancer.MethodsThe expression of E-cadherin,EGFR,AKT,c-Myc,FOXO3 a,and ATP4 b in the gastric cancer tissues and adjacent tissues of 53 patients were detected by immunohistochemistry(SP)method,and the relationship between the expression level and the clinicopathological parameters was analyzed.The AGS cell lines of gastric cancer with overexpression of E-cadherin and low expression of EGFR were constructed,and the protein expressions of E-cadherin,EGFR,AKT,FOXO3 a,c-Myc and ATP4 b in the cells were detected by Western blot,and the correlation was analyzed.Result1.The positive expression rates of E-cadherin,FOXO3 a,ATP4b and c-Myc protein in 53 gastric cancer tissues and the corresponding paracancer tissues were higher in paracancer tissues than in gastric cancer tissues,and the expression rates of AKT protein were higher in paracancer tissues than in gastric cancer tissues.E-cadherin protein in gastric cancer is associated with the differentiation degree and staging of gastric cancer patients with pathological,EGFR protein is associated with gastric cancer patients with pathological staging,AKT protein is associated with gastric cancer tissue differentiation degree and the installment,FOXO3 a protein is associated with gastric cancer patients with pathological degree of differentiation,c-Myc protein is associated with lymph node metastasis in patients with gastric cancer,ATP4 b proteins associated with gender and pathological staging of gastric cancer patients.2.The expression of E-cadherin and FOXO3 a in gastric cancer tissues was positively correlated(r=0.376,P=0.003).There was a positive correlation between E-cadherin and ATP4 b protein expression in gastric cancer tissues(r=0.333,P=0.015).There was a negative correlation between E-cadherin and AKT protein expression in gastric cancer tissues(r=-0.292,P=0.034).There was a positive correlation between EGFR and AKT protein expression in gastric cancer tissues(r=0.285,P=0.038).3.There was a positive correlation between E-cadherin and FOXO3 a expression in gastric cancer cells(P=0.007).There was a negative correlation between E-cadherin and AKT protein expression in gastric cancer cells(P=0.025).There was a positive correlation between EGFR and AKT protein expression in gastric cancer cells(P=0.013).There was a negative correlation between EGFR and FOXO3 a expression in gastric cancer cells(P=0.037).Conclusions1.During the development and progression of gastric cancer,E-cadherin,FOXO3 a,ATP4b,and c-Myc may have tumor suppressor genes;AKT may have oncogene effects.2.E-cadherin,FOXO3 a,AKT and ATP4 b may participate in the development of gastric cancer.E-cadherin may affect the viability of gastric cancer cells by regulating AKT/FOXO3 a signaling.3.EGFR may inhibit the activity of FOXO3 a gene through PI3K/AKT signaling pathway,and participate in the development of gastric cancer,which may be a new therapeutic approach and target for gastric cancer patients.