Effect Of Sialyltransferase ST3Gal-? On Proliferation,Metastasis And Apoptosis Of Prostate Cancer Cell Line PC3

Prostate cancer is one of the common diseases that severely plagues men worldwide.The incidence of this cancer increases with age.As the disease progresses,cancer cells gradually spread to tissues or organs near the prostate,and even to parts such as lymph,brain or bone that are far away from the gland.However,the causes of the disease are not fully understood,and the genetic and epigenetic modifications that lead to tumorigenesis remain unresolved.Therefore,intensive research is still required to unravel the disease mechanism of prostate cancer.During carcinogenesis,the amount of intracellular glycosyltransferase expression changes,including sialyltransferase.The abnormal expression of sialyltransferase modifies the structure of the sugar epitopes on the surface of cells,leading to the inability to be recognized by immune cells and promoting the pathological characteristics of cancer cells in terms of metastasis.In this paper,the effects of ?2-3 sialyltransferase(ST3Gal-?)on the migration,proliferation and apoptosis of prostate cancer PC3 cells were investigated.The main contents of this research are as follows: 1.Difference in sialic acid content between cancerous tissue and adjacent normal tissuesIn this study,the patient's cancer tissues and the adjacent normal tissues were treated via tissue lysate and acid hydrolysis,and the sialic acid in the tissue was released,and the protein was precipitated by the trichloroacetic acid method.The sialic acid concentration was determined by measuring the absorbance of sialic acid via the thiobarbituric acid method.Verify whether there is a difference in sialic acid content between cancerous and normal tissues 2.In vitro study of the effect of sialyltransferase on proliferation,migration and apoptosis of PC3 cellsFirstly,the expression of ST3Gal-? in PC3 cells was knocked dwon via siRNA and transfection technology and the transfection conditions were optimized.The proliferation rate of PC3 cells after transfection was determined by MTT assay.PC3 Lectin staining assay was employed to determinethe changes of the sugar chain structures on the cell surface,and the expression levels of apoptotic receptors were determined by the fluorescently linked antibody via flow cytometry.The results showed that ?2-3 sialyltransferase was involved in the synthesis of sialic acid-related sugar chains on the cell surface,whichaffected the proliferation,migration and apoptosis of PC3 cells.3.In vivo study of the effect of sialyltransferase on the tumorigenic ability of PC3 cell lineTumor models were established using nude mice to investigate the difference in tumorigenic ability between the siRNA transfected PC3 and the untransfected and control siRNA transfected PC3 cell lines.Western blot analysis was used to determine the expression of targeted protein after trasnfections.As death receptors DR4/5 are glycoproteins,their expressions were also determined.The results showed that when the expression of ?2-3 sialyltransferase was interfered,and expression of DR4/5 were not affected,the tumorigenic ability of PC3 cell line was regulated,and the formation of tumors showed reduced volume in the comparison to the un-transfected PC3 cell line.In summary,both in vitro and in vivo experiments have demonstrated the effects of ?2-3 sialyltransferase on the proliferation,migration and apoptosis of prostate cancer cells,suggesting that elevation of ST3Gal-? expression promotes prostate cancer progression and is a potential target for cancer therapy.