The Value Of CD97 And CD55 In Differential Diagnosis Of Tuberculous Pleural Effusion And Malignant Pleural Effusion

Objective:To study the application value of protein CD97 and CD55 in differential diagnosis of tuberculous pleural effusion and malignant pleural effusion.Objects and methods:The patients with tuberculous pleural effusion and malignant pleural effusion at the Department of Respiratory Medicine,First Hospital of Jilin University from April 2017 to November 2018,as a research object.After strict screening,a total of 106 patients were included in the study.There were 62 males and 44 females with an average age of 55.96±16.92 years old.Patients enrolled in the study were divided into two groups,tuberculous pleural effusion group and malignant pleural effusion group.There were 55 patients with tuberculous pleural effusion group,including 34 males and 21 females,with an average age of 48.81±18.71 years.There were 51 cases in the malignant pleural effusion group,including 28 males and 23 females,with an average age of 62.82 ±10.90 years.General clinical data and laboratory examination data were collected,including erythrocyte sedimentation rate and c-reactive protein,the initial pleural effusion obtained by thoracoscopy or pleural effusion puncture and drainage was collected.The supernatant was obtained after the specimen was processed.The concentrations of CD97 and CD55 in pleural effusion were detected by ELISA.At the same time,the concentration of TNF-?and IL-1?in the pleural effusion of the two groups was detected by ELISA.Than we will analyz the differences of CD97 and CD55 levels between the two groups.ROC curve was made to clarify the sensitivity and specificity of CD97 and CD55 and their combination for differential diagnosis.To study the correlation between CD97 and CD55 and inflammatory indexes in each group,the correlation between CD97 and CD55 in the two groups.Result:1?The concentrations of CD97 in TPE group and MPE group were 161.80±66.46ng/ml and 111.97±52.20ng/ml,respectively.The CD97 level in TPE group was significantly higher than that in MPE group,and the difference was statistically significant(P<0.001);The concentrations of CD55 in TPE group and MPE group were64.21±19.89ng/ml and 116.22±40.46ng/ml,respectively.CD55 level in MPE group was significantly higher than that in TPE group,and the difference was statistically significant(P < 0.001).2?ROC curve indicated that cut-off values of CD97 and CD55 in differentiating tuberculous pleural effusion from malignant pleural effusion were 105.26ng/ml and 79.05ng/ml,respectively.The area under the curve was 0.724 and 0.917,respectively.The sensitivity and specificity of CD97 in differentiating tuberculous and malignant pleural effusion were 80.0% and 60.8%,respectively.The sensitivity and specificity of CD55 in differential diagnosis between the two groups were 88.2% and85.5%.The area under the curve,sensitivity and specificity in the diagnosis of tuberculous and malignant pleural effusion were 0.922 80.4%and 92.7%,respectively.3?In MPE group,CD97 is negatively correlated with CD55(r=-0.383 p=0.005).In the TPE group,TNF-?was positively correlated with IL-1?(r=0.766 p<0.001).In MPE group,TNF-? was positively correlated with IL-1?(r=0.568 p<0.001).There was no correlation between CD97 and CD55 in TPE group.There was no correlation between CD97 and CD55 in TPE group and MPE group and various inflammatory indicators(TNF-?,IL-1?,ESR and CRP).4?The expression of CD97 and CD55 in pleural fluid was not related to gender.The ratio of CD97 and CD55 in male and female groups was greater than 0.05,which was not statistically significant.Conclusion:1?CD97 and CD55 can be used as biomarkers for the identification of tuberculous pleural effusion and malignant pleural effusion.2?CD97 and CD55 were negatively correlated in malignant pleural effusion.