A Study Of Gastric Cancer Cell Line MGC803 Apoptosis Induced By Arsenic Trioxide

Gastric cancer is a common malignancy and seriously threatens human health.Chemotherapy is an important method for treating gastric cancer.It is of great value to develop new chemotherapeutic drugs for gastric cancer.As₂O₃ can inhibit gastric cancer cells proliferation and induce gastric cancer cells apoptosis,but the precise mechanism is still unclear.In this study,we investigated gastric cancer cell line MGC803 apoptosis induced by As₂O₃.Cell proliferation assay showed that As₂O₃ could inhibit MGC803cells proliferation in a time-and dose-dependent manner.We used IC50 concentration of72h?0.4mg/L?as experimental dose,and then carried out cell count assay,cell scratch assay,cell cycle assay,cell apoptosis rate assay,cell mitochondrial membrane potential assay,intracellular Caspase-3 protein assay and DNA ladder assay.The following results were obtained:1.The number of viable cells decreased in the test group while increased in the control group.2.Cell scratch area had little changes in the test group while it decreased significantly in the control group.3.For the test group,the apoptotic rate increased continuously,the cell mitochondrial membrane potential decreased significantly and the proportion of G₂/M phase increased.4.Caspase-3 content of the test group was significantly higher than that of the control group,and the trend was more obvious with time elapsing.5.Typical trapezoidal bands appeared in the test group.Then we used FTIR spectroscopy to investigate biochemical changes?proteins,lipids,nucleic acids,etc.?of MGC803 cells apoptosis induced by As₂O₃,and the following results were obtained:1.For the test group,proteins and lipids structure changed,and protein content increased relative to the lipid content.2.PCA showed that the structure/content of nucleic acid changed after As₂O₃ action significantly.The above results indicated that As₂O₃ could inhibit MGC803 cell growth and migration,block cell cycle at G₂/M phase,and induce MGC803 cells apoptosis through reducing mitochondrial membrane potential,producing Caspase-3 and breaking cell DNA.In addition,FTIR spectroscopy could obtain biochemical changes of MGC803 cells apoptosis induced by As₂O₃ at molecular level,which would be a useful supplement for further understanding of apoptosis mechanism.