Preparation And In Vitro Study Of Biomimetic Multifunctional Guided Bone Regeneration Nanocomposite Membrane

Objective To construct a three-dimensional system of shell-like layered double-layer composite film,and to characterize its structure,test its mechanical properties,biocompatibility,bacteriostasis,cell adhesion and in vitro degradation ability,and explore its role as a new GBR.Feasibility of membrane materials.Methods Chitosan(CS)is an important tissue engineering scaffold material.Graphene oxide(GO)is an important element for constructing shell-like structures.Calcium silicate(Ca Si)is the most important third-generation bioceramic.One.The CS solution,the GO dispersion and the Ca Si dispersion were mixed in a certain ratio,ultrasonically crushed and dispersed,and the film was formed by self-evaporation,and then the porous layer structure was prepared by the ice template method.The microstructure was observed by SEM and TEM.The composition of the material was characterized by XRD.The tensile properties of the composite membrane were tested by the mechanical universal testing machine.The cell compatibility of the composite membrane was detected by CCK-8 method.Staphylococcus aureus,Escherichia coli and transformation were selected.Streptococcus was tested for bacteriostasis,human dental pulp mesenchymal stem cells were selected for cell adhesion experiments,and lysozyme was selected for in vitro degradation experiments.Results After adding GO and Ca Si,the lower layer of the composite membrane has an ordered layered structure,and the upper layer is a rough porous structure.The tensile strength is obviously improved,and it can reach 152.5 MPa in the dry state,which is Bio-Gide membrane 40 times up to 11.6 MPa in wet state,10 times that of Bio-Gide membrane.The CCK-8 experiment showed that the material was not cytotoxic.The bacteriostatic experiment showed that the composite membrane inhibited the growth of Staphylococcus aureus,Escherichia coli and Streptococcus mutans.The cell adhesion experiment showed that the cell adhesion of porous surface was significantly increased.On a smooth surface,in vitro degradation shows that the composite membrane is degradable.Conclusions The composite film with mass fraction of 4% GO and 5% Ca Si has the best tensile strength,which is much higher than the most widely used Bio-Gide collagen membrane,and the composite membrane has good cytocompatibility,antibacterial property and Degradability lays the foundation for further in vivo experiments and the development of a new generation of GBR membranes.