Differential Expression Of Septin2 In Colorectal Cancer And Its Correlation With Clinical Pathological Data

ObjectiveThis study was to investigate the abnormal expression of GTP-binding protein benzyl chloride 2(Septin2;SEPT2)in colorectal cancer and its relationship with clinicopathological data.Methods1.The expression of Septin2 mRNA in colorectal cancer tissues was investigated by seeking the Oncomine public database.2.The protein content of colorectal cancer tissues was detected by Western blot.The pathological sample we selected was the first hospital affiliated of Jinzhou Medical University in September 2018.Patients with colorectal cancer surgery did not receive preoperatively,such as radiotherapy,chemotherapy,with out distant metastasis.3.The Septin2 immunohistochemical staining score(IRS)was evaluated and recorded by immunohistochemistry(IHC)staining method,and the expression difference of IRS in different pathological data was analyzed;Purchase colorectal cancer tissue chip(Shanghai Outdo Biotech;Chian)(chip number:HColA180su14),with the chip we obtained the patient’s clinical pathological data and overall survival of patient.4.Using SPSS20.0,based on IRS,pearsonχ~2 test was used to test the abnormal expression of colorectal cancer.The pearsonχ~2 test was used to compare the IRS difference between different clinical pathological parameters.The survival curve was drawn by Kaplan-Meier method,and the statistical difference of overall survival was evaluated by log-rank test.Univariate analysis and multivariate analysis were performed to assess the risk ratio of clinical factors that may have an impact on overall survival.Results1.The Oncomine public database showed that at the mRNA level,Septin2was expressed in colocectal cancer tissues higher than normal tissues.2.Western blot results showed that the protein content of Septin2 in colorectal cancer tissues was higher than that in paracancerous tissues.3.After statistical analysis,the IRS of Septin2 in colorectal cancer tissues is different from that in adjacent tissues.4.The results of Septin2 in colorectal cancer with IRS in different clinicopathological data showed that the IRS results of Septin2 was related to colorectal cancer cell grading,lymph node metastasis,TNM staging,while gender,age,tumor size,tumor growth pattern,T staging and distant metastasis was not significant.5.Survival curves of the Septin2 high expression group and the low expression group showed that the overall survival of the Septin2 low expression group was higher than that of the Septin2 high expression group.ConclusionsThe mRNA and protein content of Septin2 is abnormally expressed in colorectal cancer tissues and it is an oncogene closely related to the occurrence,progress,prognosis and survival of colorectal cancer.