The Role Of Biotin In Liver Steatosis Induced By Oleic Acid In Rats

Oleic acid(OA)is a monounsaturated free fatty acid with the highest content iin non-alcoholic fatty liver(NAFLD)serum.A large number of literatures have shown that oleic acid could induce steatosis in many cells,so it is often used as an inducer of the in vitro model of NAFLD.Biotin(BIO)is a water-soluble vitamin,which plays an important role in fat metabolism.In this experiment,a NAFLD model was established by oleic acid-administered SD rats,and biotin was added to the drinking water to investigate the role of oleic acid and biotin in the hepatic steatosis of rats,which provided a new idea for the establishment of NAFLD model and prevention of NAFLD.1.Effects of oleic acid and biotin on hepatic steatosis in rats32 healthy SD rats were kept in balanced diet for 1 week,and then were randomly divided into four groups:control group(0.5 mL DDW gavage),oleic acid group(0.5 mL OA gavage),oleic acid plus biotin group(0.5 mL OA gavage+40 mg/L BIO drinking water),biotin group(40 mg/L BIO drinking water).After continuous treatment for 4 weeks,the body weight of the rats was weighed,the amount of drinking water was calculated,and then blood,urine and liver tissue were collected after slaughter.The contents of ALT,AST,ALP,ALB,CHOL,LIP,GLU and TG were detected by Blood biochemical analyzer;Morphological changes of liver tissue observed by pathological section.The results showed that the body weight of the oleic acid group decreased significantly,the water intake increased significantly,and the liver to body weight ratio increased significantly(P<0.05),compared with the control group.The contents of AST,ALP,LIP,TG increased significantly or extremely significantly(P<0.05 or P<0.01);Tissue sections showed a large amount of macrobubble-like steatosis in hepatocytes.Compared with the oleic acid group,the body weight and water consumption of the oleic acid plus biotin group increased;the contents of AST,ALP,CHOL and TG decreased significantly(P<0.05);the hepatocyte macrobubble-like steatosis was less.The results showed that oleic acid-administered SD rats could cause liver steatosis,and biotin played a protective role in steatosis.2.Effects of oleic acid and biotin on key proteins in rat fat synthesisEnzyme-linked immunosorbent assay kit was used to detect the contents of key proteins VTN,CLU,F2 and LCN2 in rat urine.Western Blot was used to detect the expression of FAS and SREBP-lc in liver tissue.The results showed that the levels of VTN and LCN2 were extremely significantly increased(P<0.01),the contents of CLU and F2 were extremely significantly decreased(P<0.01),and the expressions of FAS and SREBP-lc were significant or extremely significantly increased(P<0.05 or P<0.01)in the oleic acid group,compared with the control group.Compared with the oleic acid group,the levels of VTN and LCN2 were significantly or extremely significantly decreased(P<0.05 or P<0.01),the contents of CLU and F2 were significantly increased(P<0.05),the expression of FAS and SREBP-lc was significantly or extremely significantly decreased(P<0.05 or P<0.01)in the oleic acid plus biotin group.The results showed that oleic acid could cause the changes of fatty liver key proteins in rat urine and increased the expression of key proteins in fat synthesis,further verifying the occurrence of fatty liver and the protective effect of biotin.