Preliminary Screening Study Of The Differentially LncRNA-mRNA Expression In Craniopharyngiomas

Objective:Adamantinomatous craniopharyngiomas(ACPs)are common benign epithelial tumors which are clinically invasive.Research from the recent years has become established that mutations in CTNNB1,leading to the over-activation of the WNT/?-Catenin pathway,underlie the molecular aetiology of human ACPs.However,there are few researches on tumorigenesis and progression about the pathological process of ACPs in LncRNA.Therefore,this study intends to construct the LncRNA-mRNA expression spectrum of ACPs by next sequencing technologies and then screen the LncRNAs with significant differences in ACPs.We predict the target genes of the LncRNA with the ACPs to lay the foundation for revealing the molecular mechanism of ACPs in the pathogenesis and invasive growth.It can provide new ideas for early diagnosis,prognosis and therapeutic strategies.Methods:We collected sixteen cases(the core substantive tumor tissues and surrounding tumor envelopes)from February 2018 to January 2019 diagnosed as ACPs in the neurosurgery of the First Affiliated Hospital of Nanchang University.Three pairs of the tissues were randomly selected to use next sequencing technologies to analysis with the prominent differences between LncRNA and mRNA expression.We use the bioinformatics to analyze the GO and KEGG of these LncRNAs which were screened with significant differences in ACPs.Then,according to the microarray results and literature reports,we selected six out of significantly different LncRNAs to verify the expansion using qRT-PCR.Further we analyzed the correlation between genes expansion and clinical features of the ACPs.At the same time,Western Blot was used to detect the content of ?-Catenin in the core substantive tumor tissues and surrounding tumor envelopes of ACPs.Result:There were series significant differential expression of LncRNAs and mRNAs between the core substantive tumor tissues and peripheral envelope tissues by next sequencing technologies.The results showed that there were 53 up-regulated and 178 down-regulated LncRNAs;1291 up-regulated and 3747 down-regulated mRNA(fold-change>2.0 and P-value<0.05)in core substantive tumor tissues with surrounding tumor envelopes.Using the qRT-PCR to test the 6 significant different LncRNAs in screening,the expression level of LncRNA CRNDE was elevated,and the expression level of LncRNA SNGH14?ZEB1-AS1 was decreased in the core substantive tumor tissues which was consistent with the results of next sequencing technologies(P<0.05).Although the expression of LncRNA ZIC1?FEZF1-AS1 was not same in different regions,the differential expression was not obvious(P>0.05).LncRNA MIAT did not show significant differences in different regional organizations.The expression of ?-Catenin protein in surrounding tumor envelopes was significantly higher than that in the core tumor tissue of ACPs(P<0.05).Conclusions:There are many differentially expressed LncRNA and mRNA in the core substantive tumor tissues and surrounding envelope tissues of ACPs.We found that the LncRNA CRNDE is up-regulation in the core substantive tumor tissues and the IncRNA SNGH14?ZEB1-AS1 is down-regulation in the core substantive tumor tissues which are not connected with the age?gender of patients and the size of the tumor.Meanwhile,the content of ?-Catenin protein was higher in surrounding tumor envelopes.Therefore,differential expression of LncRNAs may regulate the expression of ?-Catenin protein in different regions which may be related to the occurrence and invasiveness of ACPs.In conclusion,there may be different expressions of genetic information in different regions of ACPs.