Clinical Characteristics Of Patients With Guillain-Barre Syndrome Associated With Infection Of Campylobacter Jejuni In Southwest Of Shandong Province

Objective: To understand Campylobacter jejuni(CJ)in Guillain-Barre syndrome(GBS)patients and non-GBS by detecting antibodies related to CJ in the blood of patients with GBS and non-GBS populations in southwestern Shandong Province The infection status in the population was analyzed from the specific manifestations of GBS patients after CJ infection in southwestern Shandong,and compared with the clinical features of GBS patients with CJ infection reported at home and abroad,and the patients with CJ infection-related GBS in southwest Shandong were summarized.The specificity provides a basis for the diagnosis and treatment of clinically relevant CJ infection-related GBS.Methods: Serum samples from the experimental group and the control group were detected by an ELISA test method using an immunoassay kit.The data results were statistically analyzed using SPSS 20.0 and plotted with GraphPad Prism 6.0.The chi-square test was used to compare the clinical features of patients with CJ-related GBS who have been reported in southwestern Shandong and at home and abroad(GBS study in northern China in 1995,GBS study in the Netherlands in 2001,and GBS study in Bangladesh in 2015).Results: In this experiment,a total of 150 patients in the GBS group,non-GBS neurological disease control group(OND group)and healthy control group(HC group)were collected from southwestern Shandong.According to statistical analysis,there was no difference in the number of cases,gender,and age of the GBS group,the OND group,and the HC group(P>0.05);suggesting that the three groups were comparable.ELISA results: The number of positive CJ infections in the serum of GBS group,OND group and HC group were 40,12 and 18 respectively.The results of chi-square test indicated that GBS group and OND group(P<0.05),GBS group and HC group(P<0.05),OND group and HC group(P>0.05),indicating that CJ infection was only and GBS The incidence is related.According to the chi-square test,the results of gender ratio,clinical classification,pre-infection symptoms and cranial nerve damage were significantly different between patients with positive CJ infection and CJ infection(P<0.05).There was no difference in protein-cell separation between muscle involvement and Cerebrospinal fluid(CSF)(P>0.05);T-test analysis of two independent samples showed that GBS patients with positive and negative CJ infection were at age There were differences(P<0.05).The specific results are as follows: the proportion of male and female patients with positive CJ infection is roughly the same,the age of patients is about 43 years old;the clinical manifestations are more common in AMAN,followed by AIDP,and the incidence of Miller Fisher syndrome(MFS)is lower.The incidence of diarrhea before the onset of the disease is higher than that of the respiratory tract;the cranial nerve and the respiratory muscle are less involved;the neurophysiological examination suggests that the patient has peripheral nerve damage and is significantly damaged by the axonal cord;The case of protein-cell separation in CSF;the detection result of serum ganglioside antibody in patients is positive for anti-GM1 and anti-GD1 a antibodies.The GBS patients with negative CJ infection were more male than female.The age of the patients was about 54 years old,which was later than that of patients with positive CJ infection.The clinical manifestations were more common in AIDP type,followed by AMAN,and the incidence of MFS was higher.The former;the infection rate of the respiratory tract before the onset of the disease is higher than the incidence of diarrhea;the cranial nerve involvement is more than the GBS patients with positive CJ infection;the respiratory muscles are less involved;the neurophysiological findings suggest that the peripheral nerve is damaged,and More suggestive demyelination changes;patients with CSF protein-cell separation;patients with serum ganglioside antibodies were anti-GD1 b,anti-GalNAc-GD1 a,anti-GQ1 b antibody positive.Conclusion: CJ is closely related to the occurrence of GBS.Patients with CJ-related GBS in southwestern Shandong showed more impaired motor function,that is,mostly in the form of AMAN.Patients with limb muscle strength,decreased muscle tone,reduced or disappeared tendon reflexes;most patients had a history of diarrhea before onset;CSF multiple cue protein-cell separation;electrophysiology suggests peripheral nerve damage,and axonal damage is obvious;the test results of serum ganglioside antibody in patients with anti-GM1,anti-GD1 a antibody positive.In this study,150 patients with GBS were compared with 129 patients with GBS in the study in northern China in 1995: the positive rate of CJ infection was 26.49%,the latter was 36.56%(P>0.05),and the AMAN form accounted for CJ positive group.42% compared with the former and 42.5% of the latter(P>0.05);this indicates that the results of this experiment are basically consistent with domestic research reports.