| Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide,which seriously affects human health.The prevention and treatment of gastric cancer in China is characterized by high morbidity and mortality,low early diagnosis rate(less than 10%),low surgical resection rate(less than 70%),and low5-year survival rate(about 40%).At present,the treatment of early gastric cancer in clinical practice is a comprehensive treatment mainly based on surgical resection,while the treatment of advanced or advanced gastric cancer is palliative treatment of combined chemotherapy,radiotherapy,targeted therapy,immunotherapy,traditional Chinese medicine treatment and other treatment means.Gramicidin is a peptide antibiotic that is used to treat infections caused by bacteria or fungi.The purpose of this study was to investigate the inhibitory effect of Gramicidin on cell cycle and apoptosis of gastric cancer cells and its mechanism.Gastric cancer cell sgc-7901,gastric cancer cell bgc-823 and normal gastric mucosa cell GES1 were treated with different concentrations of Gramicidin respectively.The experiment of cell colony formation showed that Gramicidin significantly inhibited the proliferation of gastric cancer cells,but had little effect on normal gastric mucosa cells.CCK-8 assay showed the cytotoxic effect of Gramicidin on gastric cancer cells.In the scratch test,Gramicidin inhibited the migration of gastric cancer cells.In cell cycle and apoptosis experiments,Gramicidin induced cell cycle G2/M arrest by down-regulating p-FoxO1 and cyclinD1,and down-regulating bcl-2 induced cell apoptosis.The results of the experimental study on the anti-tumor effect of Gramicidin on gastric cancer cells provide a possibility for the application of Gramicidin in clinical practice as a new drug for the treatment of gastric cancer. |
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