Analysis Of The Cell Content,Components And Activity Of Nano-fat

Objective To study the difference of cell content,components and activity of Nano-fat and SVFs.Methods There are two groups.Group one is Nano-fat group.The method of preparation of cell suspension were as follows:20mL adipose tissue was mechanically emulsified between two 10mL syringes connected by a female to-female Luer-Lok connector and was then processed by centrifugation at 1200g for 3min,the remaining substance under the oil layer was digested with 0.25%collagenase I to obtain the cell suspension.Group two is SVFs group.Adipose tissue was digested directly with 0.25%collagenase I to obtain cell suspension.Before the culture,those obtained cells of two group were examined for surface marker expression and counted for cell numbers.Cultured cells were examined for surface marker expression and histology on different days.Results Mean cell number of Nano-fat group and SVFs group was 1.85±0.73×106 cells/mL and 5.57±2.63×106 cells/mL respectively(p<0.05).Flow cytometric analysis revealed adipose-derived stromal cells(CD45-/CD31-/CD34+)of Nano-fat group and SVFs group was account for 19.92±5.7%and 30.7±8.7%respectively(p<0.05).Endothelial cells(CD45-/CD31+/CD34+)was account for 50.93±10.6%and 1.94±0.6%respectively(p<0.05).Hematopoietic cells(CD45+)was account for 15.5±8.5%and 45.7±10.5%respectively(p<0.05).After culture,two group have the same trend of growth and differentiation.They all differentiate into adipocyte with the prolonging cultivation time.Conclusion The cell content of Nano-fat is lower than SVFs’s that obtained from the same volume adipose tissue.The proportion of adipose-derived stromal cells in Nano-fat group is also lower than SVFs group’s.But the proportion of endothelial cells in Nano-fat group is higher than SVFs groups.After culture,two group have the same trend of growth and differentiation.They all differentiate into adipocyte with the prolonging cultivation time.