Lentivirus-mediated NFATc1 Delaying Degeneration Of Articular Cartilage

Objective:to investigate the effect of lentivirus-mediated activation of T cell nuclear factor c1 on knee joint injection in rabbits.Methods:12 male New Zealand white rabbits were randomly divided into three groups(n=4),the control group was not treated,the anterior cruciate ligament was cut off and the medial meniscus was partially resected in the osteoarthritis model group.Two days after modeling,negative control lentivirus was injected into articular cavity once a week for 3 weeks.In intervention group,lentivirus-mediated NFATc1 was injected into articular cavity,the steps were the same as that in model group.The three groups of rabbits began to run after 4 days and were killed within 30 minutes after driving 500 mgs daily.Results:pathological sections and gross observations showed that cartilage damage was serious in the osteoarthritis model group,but the cartilage damage was lighter and the pathological stage was lower in the intervention group than in the other two groups.The osteoarthritis model group was significantly higher(p<0.05),but the intervention group had no significant increase compared with the control group(p>0.05).The number of matrix metalloproteinase-13 positive cells in chondrocytes was significantly higher in the model group than in the other two groups(p<0.05),but there was no significant increase(p>0.05)in the intervention group compared with the control group.Conclusion:Lentivirus mediated NFATcl injection into knee joint.It can delay the degeneration of articular cartilage.