Effect Of EIF4E On Autophagy In CD138~+ Cells Of Multiple Myeloma

Objective:To investigate the effect of eukaryotic translation initiation factor 4E on autophagy in CD138~+ plasma cells of multiple myeloma.Methods:Multiple myeloma CD138~+ plasma cells were treated with eIF4 E inhibitor 4EGI,and the changes of autophagy-related factors LC3-II and Beclin1 were detected by fluorescent quantitative PCR and western blot,the changes of cell proliferation inhibition were detected by MTT assay,and cell apoptosis was detected by flow cytometry.Results:Quantitative fluorescence PCR showed that the expression of LC3-II and Beclin1 mRNA was time-and concentration-dependent after 4EGI treatment of myeloma cells,and the expression levels of LC3-II and Beclin1 mRNA were gradually increased with the increase of 4EGI concentration and the prolongation of action time,and the differences were statistically significant(48h: LC3-?,r =0.942,Beclin1,r =0.952;80 ?g/ml: LC3-?,r =0.966,Beclin1,r =0.998);with the increase of 4EGI concentration,the expression of LC3-II and Beclin1 protein was gradually increased by Western blot(LC3-?,r =0.923,Beclin1,r =0.977);the inhibition rate of CCK-8 was gradually increased(r=0.996);the apoptosis rate of 4EGI-treated groups(23.23 ± 4.47,7.59 ± 1.67,2.03 ± 0.19)was significantly different from that of blank control group(0.03 ± 0.04)(P < 0.05).Conclusion:The inhibition of eIF4E can activate the autophagy of CD138~+ plasma cells in multiple myeloma and induce the death of myeloma cells.