| Objectives: To investigate the possible mechanism of early blood glucose elevation by measuring the changes of serum hemoglobin scavenger receptor soluble CD163 and visceral fat area inindividuals with different glucose tolerance and their correlation analysis.Methods:In the major communities of Shijiazhuang,according to the inclusion criteria and exclusion criteria,the eligible subjects(n=328)were selected.through the glucose tolerance test(OGTT experiment)into normal glucose tolerance group(NGR n=118)sugar regulation damage group(IGR n=123)newly diagnosed type 2 diabetes group(T2DM n=87).General information collected: name,gender,age,occupation,family history of diabetes,past medical history;measurement of anthropometric indicators: blood pressure,height,weight,waist circumference,hip circumference,calculated body mass index(BMI),waist-to-hip ratio(WHR)Detection of general biochemical indicators: fasting blood glucose(FBG),fasting insulin(FINS),2h blood glucose and insulin(2hINS),glycosylated hemoglobin(HbA1c),total cholesterol(CHOL),triglyceride(TG),low-density lipoprotein(LDL),high-density lipoprotein(HDL),liver function,renal function;determination of serum sCD163 concentration by enzyme-linked immunosorbent assay;detection of body fat percentage by dual-energy X-ray,Visceral fat area,Android,Gynoid,lean body mass.Insulin resistance was assessed using a steady state model: HOMA-IR = fasting blood glucose(FBG mmol/L)x fasting insulin(FIns mIU/L)/22.5.HOMA-β evaluation of pancreatic β-cell function: HOMA-β=20×fasting insulin(FINS mIU/L)/(fasting blood glucose [FBG mmol/L)-3.5].Results:1.Comparison of general conditions among the three groups: gender,age,family history of diabetes,hypertension,coronary heart disease,fatty liver,cerebrovascular disease,smoking history,drinking history,systolic blood pressure,diastolic blood pressure were not statistically significant(P>0.05);Compared with the normal glucose tolerance group,the body mass index,waist circumference and waist-to-hip ratio of the glucose-regulated group and the newly diagnosed type 2 diabetes group were significantly higher(P<0.05);There was no significant difference in body mass index,waist circumference and waist-hip ratio between the newly diagnosed type 2 diabetes group(P>0.05).2.There were differences in fasting blood glucose,2h blood glucose between the three groups(P<0.001).Compared with the normal glucose tolerance group,the glucose-regulated group had fasting blood glucose,2h blood glucose,2hINS,HbA1 c,HOMA-IR was increased,the difference was statistically significant(P<0.05);fasting blood glucose,2h blood glucose,HbA1 c,HOMA-IR,2hINS were increased in the newly diagnosed type 2 diabetes group,and the difference was statistically significant.The significance of learning(P<0.05),HOMA-β decreased,the difference was statistically significant(P<0.05);compared with the glucose-regulated group,the newly diagnosed type 2 diabetes group fasting blood glucose,2h Blood glucose,HbA1 c and HOMA-IR were increased,the difference was statistically significant(P<0.05);2hINS,HOMA-β were decreased,the difference was statistically significant(P<0.05);3.Comparison of body fat distribution between the three groups: visceral fat area was different among the three groups(P<0.05).Compared with the normal glucose tolerance group,the visceral fat area and Android of the glucose-regulated group were increased.Statistical significance(P<0.05);the visceral fat area of the newly diagnosed type 2 diabetes group was higher than that of the normal glucose tolerance group,the difference was significant(P<0.05),and there was no significant difference in Android.The visceral fat area of the newly diagnosed type 2 diabetes group was higher than that of the glucose-regulated group,but the difference was not statistically significant(P>0.05).There was no significant difference in the percentage of whole body fat,Gynoid and lean body weight(P>0.05).4.Compared with the normal glucose tolerance group,the serum sCD163 concentration in the impaired glucose tolerance group and the newly diagnosed type 2 diabetes group were significantly higher than those in the normal glucose tolerance group(P<0.05).Compared with the glucose-regulated group,the serum sCD163 concentration in the newly diagnosed type 2 diabetes group was significantly higher(P<0.05).5.Analysis of visceral fat area and pearson correlation of clinical indicators: visceral fat area and BMI,waist-to-hip ratio,systolic blood pressure,diastolic blood pressure,ALT,ALP,GGT,CREA,UA,TG,HOMA-IR,HOMA-β,HbA1 c,percentage of whole body fat,Android,lean body mass,sCD163 were positively correlated,and negatively correlated with HDL and ApoA(P<0.05).6.Pearson correlation analysis of serum sCD163 clinical indicators: sCD163 was positively correlated with age,BMI,HOMA-IR,HbA1 c,total body fat percentage,Android,visceral fat area(P<0.05).7.Taking the visceral fat area as the dependent variable,multiple linear regression analysis showed that BMI,HOMA-IR,Android,and sCD163 were independent influencing factors of visceral fat area(P<0.05).Conclutions:1.With the increase of early blood glucose,the visceral fat area increases,suggesting that the increase of visceral area may be one of the risk factors for early blood glucose elevation.2.In the early stage of blood glucose changes,insulin resistance has been clearly observed,suggesting that insulin resistance may be an important cause of early impaired glucose tolerance.3.With the increase of early blood glucose,serum sCD163 level increased,the body’s inflammatory response increased,suggesting that the body’s inflammatory response may be a risk factor for early blood glucose elevation.4.In the early stage of early blood glucose elevation,visceral fat area was independently associated with sCD163 level and HOMA-IR,and sCD163 was positively correlated with insulin resistance,suggesting that visceral fat area may further induce insulin resistance by increasing the body’s inflammatory response,leading to early blood glucose elevation. |
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