Quantitative Detection Of Atrial Virus Antigen In Patients With Corneal Endothelitis

Objective: To investigate the etiology of corneal endothelitis,To study the value and significance of real-time fluorescence quantitative polymerase chain reaction(RQPCR)in the diagnosis of corneal endothelitis in terms of etiology.Method:The antigens of herpes simplex virus,varicella-zoster virus,cytomegalovirus and EB virus were quantitatively detected in the aqueous humor of 18 patients(18 eyes),which were clinically diagnosed corneal endothelitis,We performed this experiment using real-time fluorescence quantitative polymerase chain reaction(RQPCR).At the same time,the contents of the above four virus antigens in the aqueous humor of 12 cases(12 eyes)with senile cataract were taken as the control.Results the statistical analysis was made by using the exact probability comparison of the fourfold table.Results:The aqueous humor of 18 patients with corneal endothelitis was amplified,Among them,5 cases were positive for virus antigen,and the positive detection rate was 27.78%,Among the 12 cases of control group,no case was positive for virus antigen,There was significant difference between them(P<0.05).Conclusions:The antigens of herpes simplex virus,varicella-zoster virus,cytomegalovirus and EB virus in the aqueous humor of patients with corneal endothelitis were quantitatively detected by polymerase chain reaction(PCR),A DNA level detection method can be established to achieve rapid and accurate diagnosis,and provide a reliable laboratory basis for clinical treatment.