The Epidemicology And Mechanism Research Of Cerebral Small Vessel Disease

Objective:This study is aimed to investigate the relationship between cerebral small vessel disease and cognitive dysfunction in a community-based cohort.Method:We sampled 110 people from the community and the MRI scan was conducted from 2009 to 2011.The follow-up assessment was conducted in 2011,2014 and 2017.Information on social demographic features and health status was collected and cognitive function was evaluated by Mini-Mental State Examination(MMSE)during the interview.Linear regression model was used to explore the relationship between the severity of the cerebral small vessel disease and MMSE score at baseline after adjusting for related factors.Liner mixed model for repeated mesurements was used to analyze the predicted risk factors for cognitive decline after 7 years follow-up.Result:The severity of periventricular white matter hyperintensities(WMH)was associated with lower MMSE scores at baseline,?=-0.34(-2.00;-0.24),p=0.013,after adjust for gender,age,education level,depression and the severity of cerebral infarcts,cerebral atrophy as well as subcortical white matter hyperintensities.After 7 years follow-up we found a cognitive function progressive decline in our study population.After adjust for age,gender,education level,depression and the severity of cerebral white matter lesions,cerebral atrophy and subcortical lacunar infarction,the results showed that the severity of lacunar infarction in basal ganglia was a predictive risk factor for cognitive decline in our population,p=0.020.Conclusion:The severity of periventricular WMH was related to the level of cognitive function at baseline,and the severity of basal ganglia lacunar infarction is a predictive risk factor for the progressive decline in general elder population,which provides a theoretical basis for the prevention and treatment of cognitive dysfunction.Objective:This study aimed to investigate the expression of complement receptor 1 related protein-y(crry)in cerebral amyloid angiopathy(CAA)and its relationship with A? concentration and cognitive function.We also explored the role of crry in A? clearance dysfunction after sleep deprivation.Method:APP-PS1 transgenic mice and control mice were used as animal models.Flow cytometry and immunofluorescence assay were used to detect the expression of crry in peripheral blood cells and the brian.We collected the serum and extracted the protein of cortex and hippocampus of mouse.A? concentration was determined by ELISA and water maze was conducted to detect cognitive function of mouse.We further analyzed the relationship between crry expression and A? concentration as well as cognitive function.We then examined the impacts of chronic sleep deprivation on cognitive function,A? concentration and expression of crry in APP-PS1 mouse and explore the role that crry plays in it.Result:Flow cytometry showed that crry was mainly expressed on the surface of erythrocytes and gradually decreased with the mouse aging.Compared to control mouse the expression of crry was decreased on the surface of erythrocytes in APP-PS1 transgenic mice.Further analysis showed that crry expression was correlated with A?40 concentration in cortex but unrelated to cognitive behavior.We also found that the expression of crry on erythrocytes increased after chronic sleep deprivation,suggesting that crry may be involved in A? clearance impairment in sleep disorders.Conclusion:The expression of crry on erythrocytes was significantly decreased in APP-PS1 transgenic mice and its compensatory decrease may promote A? clearance.We also found that crry expression was positively correlated with the concentration of cortical A?40,suggesting that crry might be associated with CAA lesions.What's more,chronic sleep deprivation may regulate the metabolism A? clearance by altering the the expression of crry on erythrocyte.Crry may play an important role in CAA pathogenesis.