Effect Of Injectable Bioceramic Hydrogel In Osteogenesis And The Corresponding Mechanism

[Objective]To explore the effect of akermanite/sodium alginate/glutamate combination in osteogenesis during the in vivo test of sinus floor elevation in New Zealand rabbit.Furthermore,to evaluate its osteogenic inductive effect on Human bone marrow mesenchymal stem cells(hBMSCs)and the corresponding mechanism.Besides,we aim to explore the elevated migration ability of hBMSCs stimulated by extracts of this hydrogel.[Material and methods]1.Construct the akermanite/sodium alginate/glutamate hydrogel and characterize its chemical and physical property by Fourier transform infrared(FTIR)and scanning electron microscope(SEM).Obtain extracts of this hydrogel in gradient concentrations.2.Detect the proliferation,gene expression,ALP expression,calcium deposition of hBMSCs and further investigate the corresponding mechanism3.Detect the migration ability of hBMSCs by evaluating gene expression,protein expression on membrane and migration assay.4.Evaluate the osteogenesis of akermanite/sodium alginate/glutamate hydrogel in maxillary sinus floor elevation model.[Results]1.Successfully construct the akermanite/sodium alginate/glutamate hydrogel.2.The extracts of this hydrogel promoted the osteogenesis of hBMSCs via ERK signaling pathway.Moreover,the expression of Runx2,BMP-2,ALP and BSP was greatly improved while ALP staining and ALP activity were also elevated.ARS staining reflected this trend in a more intuitive way.3.The extracts of this hydrogel improved the expression of CXCR4 on membrane according to flow cytometry.Migration assay and wound-healing test also showed this phenomenon.4.In vivo test showed that the SA/Aker/Glu hydrogel promoted the osteogenesis and angiogenesis during maxillary sinus floor elevation.And the material injected alone showed similar effect compared to its counterpart seeded with autologous cells.[Conclusion]1.SA/Aker/Glu hydrogel significantly enhanced osteogenic differentiation of hBMSCs.2.This hydrogel stimulated cell migration ability.3.This hydrogel promoted osteogenesis during In vivo test.