The Protective And Therapeutic Effects Of CQMUH-011 On Pulmonary Fibrosis In Bleomycin-induced Mice

CQMUH-011 is an Adamantane Sulfonamide derivative compound which has shown to suppress cytokines and macrophage activation and proliferation.However,its therapeutic protective and mechanism of effects in lungs fibrosis is not elucidated.In this study,we investigated and evaluated the potential protective and therapeutic effects of CQMUH-011 on Bleomycin(BLM)-induced lung inflammation and fibrosis using C57 BL mice model and mechanism involved in its effects.Results indicate that BLM-instilled C57 BL mice treated with CQMUH-011 had significant increase in weight in dose dependent manner compared with BLM only treated group.CQMUH-011 has also shown to reduce cell infiltration in the alveolar and attenuated pulmonary fibrosis as indicated in SOD activity and MDA level,collagen deposition and histopathology analysis,TNF-?,and IL-6 expression.Significantly,CQMUH-011 influenced in expression of fibrotic EMT markers E-Cadherin,?-SMA and Vimentin.CQMUH-011 was observed to enact its effect through TGF-?1-SMAD2,SNAIL and P38 signaling pathway.Furthermore,CQMUH-011 reduced mice mortality and lungs pathological damage induced by BLM in mice.In conclusion,it was suggested that CQMUH-011 have a significant antiinflammatory and anti-fibrotic effect and protect mice lungs from BLM induced inflammation and fibrosis involving in TGF-?/SMAD,SNAIL and p38 pathway.Hence CQMUH-011 may stand as a novel promising anti-inflammatory and anti-fibrotic drug.