Research On The Effect Of The Hp Eradication Therapy On Helicobacter Pylori-associated Peptic Ulcer On Gut Microbiota

Aims:Evaluate the change of gut microbiota diversity in patients with Helicobacter pylori-associated ulcer after treatment with standard quadruple therapy.Methods:We recruited 20 patients who were with active peptic ulcer with H.pylori infected without any complications randomized into a standard quadruple treatment?QT?group.20 health people were recruited as health control?HC?group.The QT group received 2 weeks of quadruple treatment.All the clinical symptoms and side effects during or after the treatment were observed and recorded.4 weeks after therapy,patients should take gastroscopy and¹⁴C-urea breath test again.We collected fecal samples at the beginning of treatment,2 and 6 weeks after the treatment?recorded as QTF1,QTF2,QTF3?.All samples were extracted V3-V4 region sequence of the 16S rDNA by PCR,and the gut microbiota was analyzed through the high-flux sequencing platform of Illumina Miseq.Results:The ulcer healing rate were 95%and H.pylor eradication rate were90%after 4 weeks anti-H.pylori treatment.There were no serious adverse reactions and complications during the treatment,the clinical symptoms were improved significantly after the treatment.16S rDNA sequencing analysis reveales the average OTUs are 872 in HC group,639 in QTF1,570in QTF2 and 615 in QTF3 group.The species diversity of QT group was lower than HC group.The treatment can lead to a decrease in intestinal flora diversity after 2 weeks.We also can find a recovery trend at 4 weeks of discontinuation.Conclusion:Gut microbiotic diversity in peptic ulcer patients is less than healthy people.Anti-H.pylori treatment resultes in continuing decline of the gut microbiota adiversity.After 4 weeks,the gut microbiota recovered slowly.