Protective Effect Of Exogenous Apelin-13 On Rats With Severe Acute Pancreatitis

Objectives The severe acute pancreatitis model was established by retrograde injection of taurocholate into the pancreaticobiliary duct to detect the changes of cytokines and inflammatory mediators in severe acute pancreatitis injury,and to explore the therapeutic effect of exogenous Apelin-13 on SAP rats and its effect on NO changes.Methods Seventy-two SPF-class SD rats were randomly divided into 3 groups by random number table,24 in each group,which were blank group,SAP group and Apelin-13 treatment group.The blank group only separated the gastroduodenum,exposed the pancreatic duct,and did not perform other treatments.In the SAP group,SAP rat model was made by retrograde injection of 5% sodium taurocholate into the pancreaticobiliary duct and the saline was injected into the tail vein.The treatment group was injected with exogenous Apelin-13 0.1 mg/kg through the tail vein of the rats.Abdominal aortic blood was collected at 3 h,6 h,12 h,and 24 h after model establishment,and pancreatic tissue was taken.Serum amylase,interleukin-6,tumor necrosis factor-? and concentration were detected by ELISA.The expression of iNOS and eNOS in pancreatic tissue was detected by protein immunoblotting,and the gross changes of HE and the pathological score of HE staining were observed.Pathological damage to the pancreas.Results 1 At the same time point,the AMS,IL-6,TNF-? and NO were increased in the SAP group compared with the blank group(P<0.05),iNOS was highly expressed,and eNOS was low(P<0.05).SAP group The histopathological scores and lesions of the pancreas were significantly heavier than the blank group.2 At the same time point,the AMS,IL-6,TNF-?,and NO were increased in the treatment group compared with the blank group(P<0.05),iNOS was highly expressed,and eNOS was low(P<0.05).Pancreatic histopathological scores and lesions were heavier than the blank group.3 At the same time point,compared with SAP group,AMS,IL-6,TNF-?,NO decreased(P <0.05),iNOS showed low expression,eNOS showed high expression(P<0.05),treatment group pancreas Histopathological scores and lesions were lighter than those of the SAP group.4 The expression of serum amylase,IL-6,TNF-?,NO,iNOS and eNOS in the blank group did not change significantly with time.The AMS,IL-6,TNF-? and NO increased gradually in the SAP group with time(P<0.05),high expression of iNOS and low expression of eNOS were more obvious;the concentration of AMS,IL-6,TNF-? and NO increased in the treatment group with a lower degree.Conclusions 1 IL-6,TNF-?,NO and other inflammatory factors play an important role in the development of SAP disease.2 Exogenous Apelin-13 can down-regulate the concentration of IL-6 and TNF-? in SAP rats and up-regulate the expression of eNOS and down-regulate the expression of iNOS.3 Exogenous Apelin-13 has a significant protective effect on severe acute pancreatitis in rats.Figure 15;Table 6;Reference 176...