Anti-oxidative Stress Effects Of Luteolin In Diabetic Rats Retina

ObjectivesTo observe the effects of luteolin on VEGF expression,SOD activity,MDA content and relative expression of HO-1 and Nrf2mRNA in Retina of diabetic rats,and to analyze the effects of luteolin on the antioxidant stress of retina in diabetic rats.MethodsUsing Sprague Dawley（SD）male rats as the object of study.Using hand-held eyeglasses to observe the front and bottom of the eye.All the rats were divided into a blank group（NC group,n=10）and a model group（n=45）by the random digital table.In the model group,rats was induced by twice intraperitoneal injection Streptozototin.After 48h,the blood sugar was measured in the tail vein of the rat and the blood sugar continued to≥16.7 mmol/L.A week later,10 were selected according to the random number table method for the control group（DM group）,10 for the low dose group（L-LUT group）,10 for the medium dose group（M-LUT group）,and 10 for the high dose group（H-LUT group）,5mg/kg/day,10 mg/kg/day,25 mg/kg/day were given to luteolin in low,medium,and high doses for 12 consecutive weeks.In the control group,DM group of 0.1%DMSO were administered with gastric administration.The random blood sugar at the tail tip of rats and weight was measured weekly after administration.In 12 weeks,the rats were executed and the eyeballs were removed.Retinal morphology was observed through hematoxylin-eosin staining.The average optical density of vascular endothelial growth factor was investigated by immunohistochemical method,and superoxide dismutase was detected by xanthine oxidase method.Content,thiobarbital acid method to measure malonaldehyde content,Real-time fluorescence quantitative accounting system method check the NF-E2 related factors,heme oxidase mRNA relative expression.Results1 The difference in blood sugar between the model group and the blank group was statistically significant（P<0.05）.There was no difference between bood sugar of each LUT group and blood sugar of DM group（P>0.05）.2 HE staining:Retinal morphology changes,ganglion cell number decrease and cytoplasmic edema in DM group.The retina of rats in each LUT group was better than the DM group,the number of ganglion cells was slightly reduced,and some cells were vacuolized.Rats of H-LUT group had clear retinal layer,ganglion cell arrangement and good morphology.3 Positive expression of VEGF was found in retina tissues of rats in each group,mainly distributed in retinal ganglion cell layer.MOD_VEGFEGF in DM group was higher than that in NC group,the difference was statistically significant（P<0.05）.MOD_VEGFEGF in each LUT group has a downward trend compared with DM group,among which the high LUT group has the best effect in reducing MOD_VEGF,and the difference is statistically significant（P<0.05）.4 SOD activity in DM group was lower than that in NC group,MDA content was higher than that in NC group,and the difference was statistically significant（P<0.05）.Compared with DM group,SOD activity increased and MDA content decreased in each LUT group.among them,high LUT group had the best effect of enhancing SOD activity and reducing MDA content,and the difference was statistically significant（P<0.05）.5 The relative expression of Nrf2 and HO-1mRNA in DM group was lower than that in NC group,and the difference was statistically significant（P<0.05）.The expression of Nrf2and HO-1mRNA was up-regulated in each LUT group compared with DM group.among them,the effect of up-regulating Nrf2 and HO-1mRNA in high LUT group was the best,and the difference was statistically significant（P<0.05）.Conclusions Luteolin can activate Nrf2/HO-1 pathway,enhance SOD activity,reduce MDA content and inhibit VEGF expression to protect retina of diabetic rats,and high dose is better.Figure14;Table13;Reference 91.