The Mechanism Of Regulating MAPK/JNK Signal Expression By PTEN Gene Silencing In Cell Proliferation Of Colon Cancer

Objective:In this study,to explore the effect of tumor suppressor gene PTEN on the carcinogenesis and molecular mechanism of colorectal cancer cells through MAPK/JNK signaling pathway.Methods:?1?The cell culture and grouping:Collect colon cancer cells SW620 into three groups,adjust the cell suspension concentration of each group to 3*10⁵cells/ml;?2?Experimental grouping and transfection:Three groups of colon cancer cells were transfected in vitro using RNA interference technology:?1?Blank control group?without any treatment?,?2?Interference empty vector group?transfected siRNA-NC?,?3?Interference expression group?transfected PETN siRNA?;?3?The three groups of colon cancer cells transfected with plasmid were cultured in an incubator for 48 h;?4?RT-qPCR to detect the contents of PTEN gene in samples of colon cancer cell line;?5?The effect of PTEN on the proliferation of colon cancer cells was detected by MTT assay;?6?Flow cytometry detection of PTEN on cell cycle in colon cancer;?7?Western blot was used to detect the expression of JNK,p-JNK,c-fos and AP-1 protein in each group.Results:?1?The results of RT-qPCR showed that compared with the interference empty vector group and the blank control group,the relative content of PTEN in the interference expression group was significantly lower,the difference was statistically significant?P<0.05?;?2?The results of MTT analysis showed that compared with the interference empty vector group and the blank control group,the proliferation rate of the interference expression group increased significantly,and the difference was statistically significant?P<0.05?;?3?Flow cytometry found that compared with the interference empty vector group and the blank control group,the number of cells in the G₂+S phase of the interference expression group increased,and the difference was statistically significant?P<0.05?;?4?Western blot results showed that compared with the interference empty vector group and the blank control group,the protein expression levels of JNK,p-JNK,AP-1 and c-Fos in the interference expression group were significantly increased,the difference was statistically significant?P<0.05?.Conclusion:1.PTEN gene silencing may promote the proliferation and DNA synthesis of colorectal cancer cell lines in vitro,so it can affect the malignant proliferation of colorectal cancer cell lines by regulating the expression of PTEN;2.PTEN gene silencing can up-regulate the expression of JNK,AP-1 and c-fos proteins,thereby activating the MAPK/JNK signaling pathway,which can promote the malignant development of colorectal cancer cell lines.