The Effect Of 1% Atropine Gel On Form Deprivation Myopia And Related Proteomics Analysis

ObjectiveThe prevalence of myopia has increased year by year,and the proportion of high myopia has increased year by year.The interventions of myopia control have become more and more important.As a previous drug used to control myopia,atropine has been corroborated by evidence-based medical medicine,but its mechanism has not been undermined yet.To explore the mechanism of atropine in controlling myopia,and to explore the effect of atropine on form-deprivation myopia.Methods1: 24 pigmented guinea pigs of 24 days old were selected,diopter ? +1.00 D,anisometropia ? 1.50 D,randomly divided into 4 groups,6 in each group,respectively: Normal refractive development(NOR)group,4 weeks of monocular Form Deprivation Myopia group(FDM),4 weeks of form deprivation and 4 weeks of atropine(F + A4)group and 4 weeks of atropine(A0)group.1% atropine gel was applied once every afternoon at 3 pm,one drop at a time.Biometric measurements were performed at baseline,two weeks,and four weeks,including refractive error,axial length,central curvature of the cornea,and intraocular pressure,etc.2: 95 24-day-old pigmented guinea pigs were selected,with diopter ?+1.00 D and anisometropia ?1.50 D,and randomly divided into 5 groups,19 in each group,respectively.There were NOR group,FDM group,4 weeks of form deprivation and two weeks of atropine(F+A1)group,4 weeks of form deprivation and 4 weeks of atropine(F+A2)group and A0 group.1% atropine gel was applied once every afternoon at 3 pm,one drop at a time.The refractive error and axial length measurements were performed at baseline,two weeks,and four weeks.After the end of the measurement,6 of each group were subjected to H&E staining;6 of each group were observed by transmission electron microscopy;7 of each group(except A0)were taken for iTRAQ proteomics analysis.Results1: The FDM group could induce-6.51 D,and the length of the axial length increases by 0.28 mm,which is statistically significant compared with the other groups(P<0.05).The F+A4 group could induce-3.76 D and the axial length increases by 0.11 mm.There were no statistically significant differences in corneal curvature and intraocular pressure between the groups(P>0.05).2: The FDM group could induce-5.95 D around the end of the experiment,the axial length increased by 0.24mm;the F+A1 group could induce-4.99 D,the axial length increased by 0.19mm;the F+A2 group could induce-3.40 D,the axis length increased by 0.13 mm.After the H&E staining,the sclera became thinner in the posterior pole of the FDM group.The collagen fibers were loosely arranged and disordered,and the thickness of the sclera was statistically different(P<0.05).Under the electron microscope,the diameter of the small fibers in the middle and outer layers of the FDM group did not change much.The diameters of the thick and thin fibers from the inside to the outside increased in other groups and all the U test showed statistical significance(P<0.05).iTRAQ proteomics found that there were 288 different proteins in the retina,of which 70 were up-regulated,67 were down-regulated by atropine,and 42 different proteins in the sclera,3 of which were up-regulated by atropine,and 7 of which were down-regulated.ConclusionsForm deprivation for four weeks could successfully induce relative myopia-6.51 D,and the axial length increases by 0.28 mm.1% atropine gel can inhibit myopia by about 42.24%,and the effect of controlling myopia is mainly in the sclera,which might do not affect other eye tissues.Proteomic analysis showed that atropine plays a role in controlling myopia and still acted on the tight junction between cells,cytoskeleton and so on.