MiRNA-122 Mimics Study On The Sensitization Of Chemotherapy In HepG2 Cells

Objective:To observe the effect of mirna-122 mimics on the growth of HepG2 cells in liver cancer,providing experimental basis for clinical treatment of liver cancer.Methods:Lab-grown HepG2 liver cancer cells.The cultured cells were randomly divided into two groups,group A as the blank control group and group B as the transfection group,namely the mirna-122 mimics group artificially transfected by HepG2 cells.The inhibition rate of cell proliferation was determined by MTT assay and the apoptosis rate was detected by flow cytometry,respectively.Results:1.Results of MTT assay:the transfection of miRNA-122 mimics had an inhibitory effect on the growth of liver cancer cells.Compared with the control group,the inhibitory rate of the transfection group was higher than that of the control group(P < 0.01).And the inhibition rate in the 24 h transfection group was greater than 48,72 hours(P < 0.05).2.Results of flow cytometry: the apoptosis rate of liver cancer cells transfected with miRNA-122 mimics was significantly higher than that of the control group(P <0.01).Conclusion:miRNA-122 mimics transfected into HepG2 cells of liver cancer can inhibit the proliferation of HepG2 cells and promote the apoptosis of HepG2 cells.