Expression And Clinical Significance Of MicroRNA-155 In The Plasma Of Patients With Primary Sj?Gren's Syndrome

Objective:To explore the role and clinical significance of microRNA-155(miRNA-155)in plasma of the patients with primary Sj?gren's syndrome(pSS)by observing its expression level in different groups and the change of clinical indexes and experimental test indexes.Methods:This study enrolled 75 pSS patients of our hospital from Jan 2017 to Dec 2018,including 41 cases without treatment(non-treatment group)and 34 cases with treatment(treatment group),and 40 sex,age-matched healthy control(health control group).We recorded symptom and various auxiliary examination indexes of patients with pSS.Then,the plasma level of miRNA-155 was test by RT-PCR in pSS and health controls,and analyze the difference of clinical and laboratory indexes between treatment group and non-treatment group.The difference of plasma level of miRNA-155 was compared among three groups:treatment group,non-treatment group and healthy control group.Moreover,we analyzed the relationship between miRNA-155 and inflammatory and experimental test indexes.All data were analysis by SPSS 24.0.Results:1.ESR and IgG level [32(23)mm/h and 18.6(9.2)g/L] in non-treatment group were significantly higher than those [14(10)mm/h and 16.6(6.9)g/L] in treatment group(P<0.01 and P<0.05).2.The plasma level of miRNA-155 was significantly lower in pSS group than in healthy control group[0.0055(0.0216)vs 0.0393(0.1926)](P<0.01).Meanwhile,the plasma level of miRNA-155 was lower in non-treatment group than healthy control group [0.0044(0.0028)vs 0.0393(0.1926)](P<0.01),and it was lower too in treatment group than that in healthy control group [0.0142(0.0700)vs 0.0393(0.1926)](P<0.05).There was no significant difference shown between non-treatment group and treatment group [0.0044(0.0028)vs 0.0142(0.0700)].3.The expression of plasma miRNA-155 in patients with pSS in high-level ESR group was higher than that in normal ESR group[0.0090(0.0345)vs 0.0033(0.0038)](P<0.05).And its expression in KSSB positive group was higher than that in KSSB negtive group[0.0228(0.0683)vs 0.0030(0.0038)](P<0.01).Moreover,the plasma level of miRNA-155 in patients co-occurred with lung disorder group was lower than those without lung disorder group[ 0.0027(0.0196)vs 0.0090(0.0207)].Patients with Chisholm III showed a higher miRNA-155 level than those with IV.[0.0142(0.0539)vs 0.0036(0.0191)](P<0.05).4.The level of ESR(r=0.630,P=0.001)and IgA(r=0.542,P=0.009)were positively related to the level of miRNA-155.Conclusion:Treatment with glucocorticoids and immunosuppressive agents would change the level of inflammation markers and IgG.The miRNA-155 level is also lower in pSS patients,and it reminds us that miRNA-155 might involved in the pathogenesis of pSS,the activation of humoral immune response,and the co-occurrence of fibrous pneumonia in pSS patients.