The Effect Of Tubeimoside-1 On The Proliferation,Metastasis And Apoptosis Of Oral Squamous Cell Carcinoma In Vitro

Objective: Tubeimoside-1(TBMS1),a triterpenoid saponin extracted from tranditional Chinese medicine Tubeimoside,exerts a cytotoxic effect on several human cancer cell lines.However,no study has focused on whether TBMS1 works on oral squamous cell carcinoma(OSCC).Therefore,we treated OSCC cells with TBMS1 to detect the effect and relevant molecular basis of TBMS1.Methods: We chose two oral cancer cell lines,CAL27 and SCC15,for this study.First,the MTT assay and Brdu assay were carried out to detect cell growth and changes in cell morphology were observed under an inverted microscope.Second,colony formation assay was performed to assess clonogenesis capacity.Next apoptosis was analyzed by flow cytometry.Subsequently,wound healing and transwell assays were applied to explore cell migration.Finally,Western blot was further performed to examine corresponding proteins’ expression change.Results: Our data showed that TBMS1 significantly suppressed proliferation of OSCC cells in a dose-and time-dependent manner and it inhibited migration of OSCC cells as well.It could also cause a marked change in the morphology of OSCC cells.After treatment with TBMS1,OSCC cells underwent cell apoptosis.Furthermore,Western blot demonstrated that TBMS1 downregulated apoptosis-associated proteins such as PARP,p-ERK1/2,Bcl-2,caspase-3,caspase-7 and caspase-8 and upregulated cleaved PARP,cleaved caspase-3 and cleaved caspase-9.It could also reduce expression of c-Myc and MMP-7.Meanwhile,TBMS1 did not change the total ERK1/2 expression.Conclusion: These results revealed that TBMS1 might be a potential chemotherapeutic drug for the management of OSCC.