Study On The Preparation Of Novel Radial Mesoporous Bioactive Glass And Its Application In Drug Delivery System

Bioactive glasses（BGs）is a kind of calcium phosphate silicate biomedical materials with good biocompatibility and biomineralization.Therefore,it has broad application prospects in the field of bone tissue regeneration.In recent years,with the development of preparation technology,researchers have introduced a template agent in the process of preparing bioactive glass by sol-gel method and can prepare mesoporous bioactive glass（MBG）with high specific surface area and porous structure.It can be used to load drugs and functional factors to improve the bone repair effect of bioactive glass.However,in the preparation of mesoporous bioactive glass in most of the template methods,there are problems such as agglomeration of bioactive glass particles and loss of calcium and phosphorus components.It leads to a decrease in biological activity and affects the research and development of MBG in the field of drug carriers.Therefore,in this paper,a mesoporous bioactive glass（MBG）with good dispersibility and high content of calcium and phosphorus components was prepared.We investigated the structure,mineralization performance,drug loading and release properties,and cytology of MBG.The main research contents and conclusions are as follows:（1）In the process of preparing bioactive glass by the sol-gel method,a two-phase layered system of cyclohexane-water is used.A curved O/W semi-emulsion micelle formed by the surfactant CTAB and an organic solvent at the oil-water interface was used as a channel template.The mesoporous SiO₂-P₂O₅ nanospheres with radial morphology（SPN）were successfully prepared by adjusting the reaction conditions.SPN has good dispersibility and large pore size,high specific surface area and particle size of about 100 nm.SPN also has a certain mineralization ability and good biocompatibility to promote cell proliferation at an appropriate concentration.（2）The prepared SPN was used as a mesoporous channel template for bioactive glass.Ca（NO₃）₂ was adsorbed in the mesoporous channel of the SPN.Mesoporous bioactive glass nanospheres（MBG）with radial morphology were prepared by high temperature solid state reaction.The effect of Ca component on the structure and properties of MBG was investigated.MBG has good dispersibility and high CaO content.It has a larger specific surface area and excellent in vitro mineralization characteristics.The material also has good biocompatibility（3）The effects of drug size and material morphology,dispersibility and composition on load performance were discussed.Aspirin was used as a drug model and BSA was a protein model.The drug release behavior of materials under different pH conditions was investigated.SPN and MBG have good loading capacity for macromolecular proteins.Materials with good dispersion and pore size matching the size of the BSA molecule have high protein loading capacity.MBG has higher protein loading capacity and better sustained release than SPN.It shows that MBG has greater advantages in drug delivery systems.