Experimental Study Of Budesonide Combined With PS In The Treatment Of Lung Injury Induced By Meconium Inhalation In Rats

OBJECTIVE: To observe whether the intratracheal instillation of budesonide and pulmonary surfactant can improve the therapeutic effect of meconium aspiration in lung injury.METHODS:By establishing a meconium aspiration model for lung injury in rats,Healthy SD rats were divided into blank control group,saline control group,meconium model group,budesonide treatment group,PS(pulmonary surfactant)treatment group and budesonide combined with PS treatment group.After tracheotomy and intubation,meconium aspiration suspension model was established by instilling meconium suspension into the trachea.Compared with the blank control group,the lung wet and dry weight,pathology were evaluated whether the modeling was successful.In the meconium model group,budesonide,pulmonary surfactant,budesonide and PS mixture were instilled into the trachea.Compared with the saline control group and meconium model group,the HE pathological changes,lung wet-to-dry weight ratio,lung function and the expression of NF-?B in lung tissue were observed.Results: 1.In the first part of the experiment,the meconium aspiration grouphad higher lung wet weight than the blank control group,and the difference was statistically significant.The pathology showed obvious destruction of lung tissue structure,alveolar collapse,hemorrhage,consolidation,etc,which prompt model established successfully 2.In the second part of the experiment,pathological changes of lung tissue,lung epithelial cell damage,consolidation,alveolar wall thickening and rupture,alveolar hemorrhage,alveolar collapse and other pathological changes were observed under HE staining pathological light microscopy.the meconium aspiration groupwas the most obvious,and the lesions in the three treatment groups were alleviated,which was significantly improved in the budesonide combined with pulmonary surfactant treatment group.3.The wet-dry weight ratio of lung tissue was highest in the meconium model group,the three treatment groups and the saline control group were lower than the meconium model group,but the difference between the groups was not statistically significant;4.Pulmonary function test showed that the tidal volume and lung compliance ofthe meconium aspiration groupwere lower than that of the saline control group,and the respiratory rate was higher than that of the saline control group.The respiratory rate of the PS group,budesonide and PS group was lower than that of the meconium model group.The lung compliance,tidal volume and ventilation per minute were higher than the meconium model group.The difference was statistically significant.The improvement of lung function was most obvious in the budesonide combined with PS treatment group;5.The expression of NF-?B in lung tissue of each group was determined by immunohistochemistry.The expression of NF-?B was significantly increased in the meconium model group,and the expression in the saline control group was low.The expression of the three treatment groups was lower than that in the meconium model group,The expression of budesonide combined with PS treatment group was the most,and the difference was statistically significant.Conclusion:1.Intratracheal instillation of 2m L/kg meconium suspension with a concentration of 25mg/m L can successfully establish a model of meconium aspiration in lung injury in SD rats.2.Intratracheal instillation of budesonide can reduce lung tissue inflammation damage to a certain extent;3.Intratracheal combined with budesonide and pulmonary surfactantalleviate lung tissue damage,improve lung function,reduce the release of inflammatory mediators to a certain extent,suggesting that it may further improve the therapeutic effect of meconium aspiration lung injury.Its mechanism may be related to the NF-?B signaling pathway.