Preparation And Motion Behavior Of Eutectic Gallium-indium Nanodroplet-Neutrophil Hybrid Motors

The self-driven micro-nano motor can convert chemical energy or other forms of energy in the surrounding environment into its own mechanical motion,and has potential application value in the field of biomedicine,especially targeted transportation.However,the current research on micro-nano motors is in its infancy,and there are still many key issues in biocompatibility and motion control.Inspired by natural immune cells,this paper studies how to use the neutrophil-specific phagocytosis and inflammatory immunochemotactic ability of E.coli and the biocompatibility and degradability of liquid metals.The liquid metal hybrid neutrophil motor is constructed to improve the biocompatibility and targeted transport capability of the micro-nano motor.The surface-stabilizer-assisted ultrasonication method can be used to control the preparation of liquid metal eutectic gallium-indium(EGaIn)nanodroplets,and to study the effects of dispersant,surface stabilizer,ultrasonic time and ultrasonic power on the morphology and particle size of nanodroplets(NDs).The results of scanning electron microscopy showed that the liquid metal EGaIn nanodroplets prepared by the dispersant was anhydrous ethanol were spherical,and the EGaIn nanodroplets were rod-shaped when the dispersant was water.The added surface stabilizer not only inhibits the formation of the oxide layer,but also determines the surface potential of the EGaIn nanodroplets.The particle size of the EGaIn nanodroplets decreases as the ultrasonic time and ultrasonic power increase.When the dispersant is anhydrous ethanol,the surface stabilizer is sodium thioglycolate,the ultrasonic time is 3 h,and the ultrasonic power is 800 W,the average particle diameter of the liquid metal EGaIn nanodroplets prepared is 116.77 ± 28.31 nm.The surface potential is-50.78 mV.A neutrophil hybrid motor modified with liquid metal EGaIn nanodroplets was prepared based on the specific phagocytosis of E.coli by neutrophils.The results of transmission electron microscopy and surface potential analysis showed that the E.coli membrane camouflage nano-droplets had a core-shell structure and had membrane components and protein orientation consistent with natural E.coli.Further co-culture of neutrophils and E.coli membranes with EGaIn nanodroplets revealed that neutrophils can actively recognize and phagocytose EGaIn nanodroplets disguised by E.coli membranes,indicating that the camouflage of E.coli membranes can be significantly enhanced.Active phagocytosis of nanodroplets by neutrophils.At the same time,it was found that the prepared neutrophil hybrid motor not only has good biological activity,but also inherits the chemotactic movement ability of neutrophils,can induce chemotaxis of chemokine responsiveness,and realize liquid metal nano liquid.Targeted transport of drops.In summary,this paper combines the biocompatibility and degradability of liquid metals with the immunogenicity and chemotaxis of neutrophils by loading liquid metal nanodroplets inside neutrophils.As a whole,this liquid metal EGaIn nanodroplet modified neutrophil hybrid motor has a good application prospect in the fields of biomedicine.