Expression Of Circular RNA HIPK3 In Lung Adenocarcinoma And Its Clinical Significance

Objective: Being covalently closed circular RNA molecules,circular RNAs(circRNAs)are widely present in a variety of biological cells and involve in gene expression regulation,with a high degree of conservation and stability.In addition,they exhibit tissue-and developmental stage-specific expression.Circular RNAs are often abnormally expressed during the pathogenesis of diseases and thus are of great diagnostic value.In lung cancers,circRNAs participate in the development and progression of lung cancers through endogenous regulation.Given that differentially expressed circRNAs are correlated with the clinical features of lung cancers,these circRNAs may serve as diagnostic markers for lung cancers.circHIPK3 is an abundant and stable circRNA expressed in various tumors,which participate in the tumor development and progression.Further investigation of the differential expression of circHIPK3 in lung adenocarcinoma is conducive to the development of novel approaches for diagnosis of lung adenocarcinoma.Methods: The 54 paired lung adenocarcinoma tissues and adjacent nontumorous tissues were collected from surgical lung cancer patients,meanwhile the clinical pathological data of the 54 patients were collected.Expression level of circHIPK3 was detected in lung adenocarcinoma and paired adjacent nontumorous tissues by quantitative real time polymerase chain reaction(qRT-PCR),differences in expression level of circHIPK3 were analyzed by the paired t-test.The relationships between circHIPK3 expression levels in lung adenocarcinoma tissues and clinicopathological features were analyzed by two sample t-test,a receiver operating characteristic(ROC)curve was established to estimate the diagnostic value of circHIPK3 in lung adenocarcinoma.Results:(1)circHIPK3 was naturally expressed in lung adenocarcinoma tissues.(2)The expression levels of circHIPK3 were lower in lung adenocarcinoma tissues than paired adjacent nontumorous tissues(P<0.01).(3)The expression levels of circHIPK3 were associated with the TNM stage(P =0.005),lymphatic metastasis(P =0.007)and tumor size(P=0.025).(4)The expression levels of circHIPK3 have diagnostic values for lung adenocarcinoma tissues.The area under the ROC curve was 0.831(P<0.01),the sensitivity was 64.8%,the specificity was 88.9%.Conclusion:circHIPK3 was downregulated in lung adenocarcinoma tissues.The expression levels of circHIPK3 have a correlation with the pathological features of lung adenocarcinoma.circHIPK3 has certain value for the diagnosis of lung adenocarcinoma.