The Role Of NRP1–FYN Interaction In Odontoblast Differentiation Of Dental Pulp Stem Cells

Objective To observe the effect of NRP1–FYN interaction in odontoblast differentiation of dental pulp stem cells(DPSCs).Methods Neuropilin-1(NRP1)is one of the members of neuropilin family.It can combine with disparate ligands involved in regulating cell differentiation.FYN belonging to the protein-tyrosine kinase family,which has been implicated in the control of cell growth,and the effect can be further strengthened by inflammatory factors.In this subject,we first investigated the expression and interaction of NRP1 and FYN in DPSCs.And then,we researched the effect of this complex controlling downstream signal pathway in normal or inflammation stimulated DPSCs.Finally,we analyzed the relationship between this role and odontoblast differentiation of DPSCs.Results In our studies,we verified that NRP1 can form complexes with FYN,and have the correlation changes in odontoblast differentiation of DPSCs.Therefore,we surmise that in the progress of dental caries,NRP1 interacts with FYN,by expanding inflammation and inhibition of odontoblast differentiation of DPSCs through nuclear factor-?B(NF-?B)signaling pathway.Conclusions This research will provide the molecular mechanism of inflammation factors of dental caries through activating NF-?B signal regulating odontoblast differentiation in DPSCs for finding new potential drug targets for the clinical treatment of dental caries.