| Objective and SignificanceThis thesis takes Zishengwan as the research object,and studies the preparation process of a new type of Chinese medicine-solid decoction.By comparing the yield of Zishengwan solid decoction with pills and decoction,HPLC fingerprint,ginseng The content of saponin Rg1,berberine and pachymic acid was studied.Is there any difference in the chemical composition between Zishengwan solid decoction and pill and decoction to explore whether it is feasible to improve the solid decoction dosage form of Zishengwan? Further research on solid decoctions provides the basis.MethodsThe experiment of this thesis is mainly divided into four parts.1.The first part of the preparation process of Zisheng Pill solid decoction: according to the prescription,the medicine was crushed by a 40 mesh sieve,and then added with water for stir frying.The factors affecting the preparation of Zisheng Pill solid decoction were investigated by single factor temperature,water addition and time.The optimum conditions for the preparation of Zisheng Pill solid decoction were selected,and the Zisheng Pill solid soup prepared under the condition was prepared.The agent was verified to prove the stability and feasibility of the preparation process of Zisheng Pill solid decoction.2.The second part of the comparison of the yield of different dosage forms of Zisheng Pill: extract is the total material basis of the clinical application of traditional Chinese medicine compound,comparing the yield of extract of Zisheng Pill,Zisheng Solid Pill Decoction and Zisheng Pill Decoction The differences in the rate of decoction of the active ingredients were found,and the sample t-test of the four extracts was performed to check whether there was statistical significance.3.Part ? Comparative study on the contents of ginsenoside Rg1,berberine and citric acid in different dosage forms of Zisheng Pills: Determination of Zisheng Pills,Zisheng Pill Solid Decoction and Zisheng Pills by HPLC By comparing the content of three effective chemical components in the two pills,the solid decoction and the decoction,the differences of the three components were further compared,and the three sample components of the three dosage forms were respectively subjected to paired sample t test to compare the solids of4.Zisheng Pills.Is there a statistically significant difference in the content of decoction and other dosage forms.5.Part ? Comparison of HPLC fingerprints between different dosage forms of Zisheng Pills: By comparing the HPLC fingerprints of Zisheng Pills and Zisheng Pills Solid Decoction and Zisheng Pill Decoction,three dosage forms can be obtained from the overall chemical composition.A comparison was made to see if the three dosage forms were different in composition.At the same time,ten batches of different batches of samples were selected for the three dosage forms for fingerprint comparison,and whether there were differences in the composition of the samples.ResultsThe factors affecting the preparation process of Zishengwan solid decoction mainly include temperature,liquid-to-liquid ratio and boiling time.The optimum conditions for the preparation of Zishengwanl solid decoction are 160 °C,liquid-to-liquid ratio 1:1,boiling time.17 min.The optimum preparation conditions were verified.The extract yield,ginsenoside Rg1,citric acid and berberine RSD were 0.43%,0.83%,0.23% and 0.44%,respectively.Comparing the yields of Zisheng Pills,Zisheng Pills and Zisheng Pills,it was found that there was no significant difference in the extraction yield between the three and Zisheng Pill solid decoction(P>0.05).HPLC method was established for the determination of ginsenoside Rg1,berberine and citric acid in Zisheng Pills,Zisheng Pills and Shisheng Pills.The regression equation of ginsenoside Rg1 is Y= 1155.4440X+32.7411,r =0.9998(n=5),the linear range of ginsenoside Rg1 is good in the relationship of 17.73?g~354.6?g.The regression equation of tannic acid is Y=1053.8127X+22.0912,r=0.9997(n=5),and the linear range is good in the range of 11.48?g~229.6?g.The regression equation of berberine is Y=1067.3493X-7.3730,r=0.9996(n=5),and the linear range is good in the range of 17.77?g~355.4?g.The average recovery rates of the three were 100.46%,99.78%,and 99.7%,respectively.The content of ginsenoside Rg1 in Zisheng Pills was 1.25 ± 0.07767,the content of ginsenoside Rg1 in Zisheng Pills was 1.66±0.01528,and the content of ginsenoside Rg1 in Zisheng Pills was1.71±0.01528.The content of 1.70±0.06658,the citric acid content of Zisheng Pill Solid Decoction is 1.03±0.03055,the citric acid content of Zisheng Pills is 1.04±0.03055,and the berberine content of Zisheng Pill is 1.20±0.06658.The berberine content of the pill soliddecoction was 1.42 ± 0.04163,and the berberine content of the Zisheng Pill was 1.39 ±0.03055.Fingerprints of four dosage forms were established by HPLC.It was found that the composition of Zisheng Pill Solid Decoction and Zisheng Pill Decoction was similar in composition,but there was a difference between the ingredients of Tongrentang Zisheng Pill.ConclusionThe experimental results show that the preparation process and brewing process of the solid decoction of Zisheng Pill are stable and convenient.The extract yield of the solid decoction prepared under this condition was not significantly different from that of Zisheng Pill and Zisheng Pill.Chemical composition,Zisheng Pill solid decoction and Zisheng Pill decoction have higher similarity,but there are certain differences in chemical composition.Zisheng Pill solid decoction and pills and decoction in ginsenoside Rg1,berberine and Compared with the content of tannic acid,the solid decoction content is similar to that of the decoction and higher than the content of the bolus. |
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