The Study Of High Fat Diet On Hepatic Lipid Metabolism On Mice And Its Mechanism

Objective: Nonalcoholic fatty liver disease(NAFLD)has become the most common chronic liver disease,which is related to various systemic diseases,causing serious harm to human health.There is closely relationship between diet and the development of fatty liver disease.This study was aimed to investigate the effects of oil consumption habits of some high fat diet on blood lipids,liver fat and uric in mice models.This research prospective evaluated the high fat diet damaged the health and its potential risks,provided the reference for rational use of oil.Method: It was designed to simulate to the high level daily oil intake(95g/d)of our country residents which is a small part(5%-10%),and it converted into 10.5% of the total oil addition in the diet.The fifty mice were randomly divided into the following 5 groups: L(10.5% lard),SSO(10.5% sunflower seed oil),SO(10.5% soybean oil),L-SSO(10.5% blend oil of sunflower seed oil and lard),L-SO(10.5% blend oil of soybean oil and lard).After feeding by experimental diets for 12 weeks,the body fat,liver fat,liver oxidation injury,liver and kidney function and the expression of related protein were detected.Result: At the 10.5%level,the body fat rate in L group was significantly higher than SSO group and L-SO group(P<0.05),remarkable higher than SO group(P<0.01),The body fat rate in L-SSO group was significantly higher than SSO group(P<0.05),while the body fat in L-SO group was significantly higher than SO group(P<0.01).The serum total cholesterol,triglyceride and free fatty aicd level in L group was significantly higher than other four group.The content of blood glucose and serum resistin in L-SSO group and L-SO group was significantly higher than L group(P<0.05),the content of blood glucose and serum resistin in L-SSO group was significantly higher than SSO group(P<0.01).The content of blood glucose in L-SO group was significantly higher than SO group(P<0.05),while the content of serum resistin was significantly higher than SO group(P<0.01).The level of serum uric acid and the activity of xanthine oxidase in L-SSO group and L-SO group were significantly higher than L group(P<0.05),which in L-SO group were significantly higher than SO group(P<0.05).The expression of SPEBP-1c and FAS in L group were significantly higher than SSO group and SO group(P<0.05),while which was significantly lower than L-SSO group and L-SO group(P<0.05).The expression of SPEBP-1c and FAS in L-SSO group and L-SO group respectively significantly higher than SSO group and SO group(P<0.01).Conclusion:(1)At the high daily oil intake level,compared with the single plant oil(soybean oil and sunflower seed oil),the lard and the blended oil(soybean oil and lard,sunflower seed oil and lard)significantly increased the body fat,blood fat,liver adipose deposition,increase the level of body's obesity and the risk of NAFLD.(2)At the high daily oil intake level,compared with the single plant oil(soybean oil and sunflower seed oil)and the lard,the blended oil(soybean oil and lard,sunflower seed oil and lard)increased the level of serum UA and the risk of gout,the underlying mechanism is the increase in activity of XOD.(3)At the high daily oil intake(95g/d)level,the blended oil(soybean oil and lard,sunflower seed oil and lard)increased the synthesis of liver fat via enhance the expression of SREBP-1c and FAS,while lard inhibit the decomposition of liver fat via weaken the expression of PPAR? and HSL,which caused the accumulation of liver fat.