Effect Of Pseudomonas Aeruginosa-mannose Sensitive Hemagglutinin On The Prolifration Of Gastric Cancer Cells And Underlying Mechanism

Objective:To investigate the anticancer effect of PA-MSHA on gastric cancer and related mechanism.Methods:1.Four gastric cancer cell lines(NCI-N87,SGC-7901,BGC823,NUGC4)were treated with different concentrations of PA-MSHA for 24 h,48h and 72 h.MTT assay was used to examine the effects of PA-MSHA on cell proliferation and screen the gastric cancer cells sensitive to PA-MSHA.2.Flow cytometry(FCM)was used to detect the apoptosis of gastric cancer cell lines treated with different concentrations of PA-MSHA for 24 hours.3.The cell migration and invasion ability of gastric cancer cell lines were detected by wound-healing assay and Transwell assay.4.The expression level of EGFR and PTEN in gastric cancer cells were detected by western blot.After knockdown of EGFR or PTEN expression by siRNA transfection,MTT assay was used to detect the effect of PA-MSHA on the proliferation of gastric cancer cells.5.Western blot was used to detected the protein expression of related signaling pathway of gastric cancer cell lines treated with different concentrations of PA-MSHA for 24 hours.Results:1.PA-MSHA inhibited the proliferation of NCI-N87 gastric cancer cells in adose-and time-dependent manner compared with the control group(P<0.05).PA-MSHA had no significant inhibitory effect on SGC-7901,BGC823 and NUGC4 cell lines(P>0.05).2.PA-MSHA could induce apoptosis of NCI-N87 gastric cancer cells,and the apoptosis rate increased with the increase of concentration of PA-MSHA(P<0.05).3.PA-MSHA could inhibit the invasion and metastasis ability of NCI-N87 gastric cancer cells(P<0.05).4.After knocking down the expression of EGFR,there was no change in the ability of PA-MSHA to inhibit the proliferation of NCI-N87 gastric cancer cells(P>0.05).After knocking down the expression of PTEN,the ability of PA-MSHA to inhibit the proliferation of NCI-N87 gastric cancer cells was weakened(P<0.05).5.PA-MSH upregulated the expression of PTEN protein and decreased the expression of p-AKT protein while the total amount of AKT protein remained unchanged(P<0.05).Conclusions:1.PA-MSHA inhibits proliferation,promotes apoptosis and inhibits invasion and metastasis of NCI-N87 gastric cancer cells in vitro.2.The inhibitory effect of PA-MSHA on gastric cancer is through the up-regulation of PTEN protein expression,which negatively regulates the AKT signaling pathway,rather than the traditional EGFR signaling pathway.