Effect Of Fluvastatin Sodium On Proliferation,Apoptosis And PI3K/Akt/mTOR Pathway In A Human Cutaneous Squamous Cell Carcinoma Cell Line SCL-1

Objective: Cutaneous squamous cell carcinoma(CSCC)is a malignant tumor originating from the epidermis or adnexal keratinocytes,which is the leading cause of death in non-melanoma skin tumors.In recent years,the incidence of cutaneous squamous cell carcinoma in China has been increasing year by year,and the age of onset of skin squamous cell carcinoma is getting younger,which seriously affects the quality of life of patients and brings heavy physical and mental burden to people.Statins are highly specific competitive inhibitors of 3-hydroxy3-methylglutaryl coenzyme A(HMG-CoA)reductase.Since its introduction in the late 1980 s,it has been widely used in the treatment of hyperlipidemia。With the wide application of statins and the in-depth study of their mechanism of action,it has been found that statins have the potential to exert the effect of polypotent cells,which can inhibit the growth,invasion,metastasis,cell proliferation and differentiation as well as regulate the cell cycle of tumor cells.This study mainly investigated the effect of fluvastatin sodium on the proliferation,apoptosis and PI3K/Akt/mTOR signaling pathway of cutaneous squamous cell carcinoma SCL-1cells,providing theoretical basis and experimental basis for further study on the treatment of skin tumors by statins.Methods: In vitro culture of SCL-1 cell lines,the absorbance value of SCL-1 cells was determined by MTT colorimetry after 24 h,48h and 72 h treatment with 0,10,20,50 and100umol/L of fluvastatin sodium,respectively,to analyze the inhibitory effect of fluvastatin sodium on the proliferation of SCL-1 cells;Changes in apoptosis were detected by flow cytometry Annexin V-FITC/PI double-label assay,and the apoptosis induction effect of fluvastatin sodium on SCL-1 cells was analyzed;The changes of mRNA relative expressions of PI3 K,Akt and mTOR were detected by real-time quantitative fluorescence PCR(RT-qPCR),and the effect of fluvastatin sodium on this pathway was analyzed.Results: The proliferation of SCL-1 cells could be inhibited in a concentration-dependent manner and time-dependent manner by different doses of fluvastatin sodium(p <0.01).Flow cytometry showed that the early apoptosis rate of SCL-1 cells with different concentrations of fluvastatin sodium was significantly increased after 24 h and 48hinterve-ntion compared with the control group(p < 0.01)and showed a concentration depe-ndence.The mRNA expression levels of PI3 K,Akt and mTOR in SCL-1 cells cultured with different concentrations of fluvastatin sodium decreased with the increase of the concentration for 24 h,which was statistically significant compared with the control group(p<0.01).Conclusion: Fluvastatin sodium can inhibit the proliferation of cutaneous squamous cell carcinoma SCL-1 cells,induce apoptosis of cutaneous squamous cell carcinoma SCL-1cells,and inhibit the expression of PI3K/AKT/mTOR pathway.