Role Of PGRN In BeWo Trophoblast Cell Invasion And Apoptosis,and Its Effects On Preeclampsia

Objective To study the functions of progranulin(PGRN)on trophoblast cell invasion and apoptosis,and provide theoretical basis for understanding the pathogenesis of preeclampsia.Methods PGRN mRNA and protein levels were detected in the placenta tissues of patients with preeclampsia.In order to select a proper cell line,human primary trophoblast cells and three cells lines(JEG-3,BeWo,JAR)that were widely used in preeclampsia were selected to determine the expressions of PGRN by quantitative polymerase chain reaction(qPCR)and Western blotting.The proper cell line with higher PGRN expression was selected for further analysis.PGRN was silenced by specific siRNA in the selected cell line.Cell invasion and apoptosis were determined by Transwell assay and Annexin V-PI,respectively.At the molecular level,the expression and the activity of invasion related proteins MMP-2,MMP-9,and apoptotic protein Caspase-3 were also measured.Results PGRN silencing reduced the invasion but increased apoptosis in BeWo trophoblast cells.PGRN silencing reduced MMP-2,MMP-9expression,and increased Caspase-3 activity.Conclusion PGRN is indispensable for the invasion and survival for trophoblast cells.The increased PGRN expression in placenta may be a kind of compensatory mechanism of preeclampsia patients.Upregulation of PGRN may help to reduce the occurrence and development of preeclampsia.