Screening Method Of Gastric Adenocarcinoma Marker Genes Based On Next Generation Sequencing Data

Gastric adenocarcinoma is one of the common malignant tumors,including mucosal adenocarcinoma,tubular adenocarcinoma,papillary adenocarcinoma,and signet ring cell carcinoma.The incidence of gastric adenocarcinoma is high,and there is also family aggregation.More than 70% of early gastric adenocarcinoma has no obvious symptoms,so it is easy to be ignored,which is also an important reason for the high mortality rate of the disease.Early diagnosis can significantly improve the prognosis of patients and prolong their survival.At present,common diagnostic methods for gastric adenocarcinoma include endoscopic(gastroscopy)examination,B-ultrasound examination,X-ray barium meal examination,CT examination,and tissue biopsy.With the development of molecular biology technology,many molecular markers have been confirmed to participate in the occurrence and development of tumors,playing an important role in the early diagnosis,effective treatment and prognosis of cancer.Finding molecular markers of gastric adenocarcinoma is of great significance for the clinical treatment of gastric adenocarcinoma.Next-generation sequencing technology is one of the important methods for screening molecular markers in recent years.Based on the second-generation sequencing data,cancer samples and paracancerous samples can be compared to analyze the SNPs,differential expression,differential splicing and other molecular changes of genes,which provide a reference for the screening of molecular markers.In this paper,we used the next generation transcriptome sequencing data of gastric adenocarcinoma to screen the marker genes and analyze the SNPs of marker genes.The main results are as follows:1.Explore a new method for finding marker genes-ATGAS(Analysis of Gene,Transcript and Alternative Splice).The method firstly screens the differentially expressed transcripts,classifies the differentially expressed transcripts,separately filters them by different methods,and finally combines the filtered results to obtain the marker genes.Such screening methods are targeted,and the marker genes obtained by screening are more reliable;2.Using AGTAS method to analyze the transcriptome sequencing data of gastric adenocarcinoma,a total of 38 marker genes were obtained.Four marker genes were selected and verified by PCR.The results of the two marker genes were consistent with the predicted results and had further research value;3.SNP analysis was performed on the transcriptome sequencing data of gastric adenocarcinoma,and 7100 cancer sample-specific SNP loci were obtained,covering a total of 6300 genes,of which 6 genes were marker genes.It is speculated that the differential expression of the marker gene may be related to the SNP of the gene.