| Background:Cancer is one of the most serious public health problems in the world.Wound bleeding is an unavoidable problem in tumor surgery,and improper extrusion,cutting or wound protection during surgery may also cause the spread of cancer cells.Therefore,effective control of hemorrhage and maximal prevention of intraoperative tumor dissemination and metastasis are important problems in tumor surgery.Polysaccharide cellulose and its derivatives,as the commonly used hemostatic materials,mainly form a gelled material to seal and stop bleeding by quickly combining with the blood oozing from the wound surface,and at the same time,it can activate the coagulation pathway and accelerate the wound hemostasis.Most of the commonly used anticancer chemotherapy drugs are intravenous infusion or oral administration,but all of them have problems such as low local blood concentration,hepatorenal toxicity and gastrointestinal side effects.However,there is no biodegradable composite material with both local anti-tumor and hemostasis efficacy in clinical practice.It has been reviewed by professional institutions,and no related reports and patent authorization have been found.Objective: This study focuses on wound bleeding after tumor resection,and residual tumor and(or)patients at risk of disseminated,research and development of a new type of antitumor active ingredient of functional materials,and evaluate its degradation in vivo and in vitro,on rat liver bleeding wound model of topical hemostatic performance and acute systemic toxicity biocompatibility and biological safety.Methods: Carboplatin biodegradable hemostatic materials were prepared by a complex cross-linking and freeze-drying method,including the processes of dissolution,cross-linking,ultra-low temperature freezing and sublimation drying of anticancer drugs and biological cellulose.Mount the carboplatin biodegradable hemostatic materials implanted in SD rats spine subcutaneous fascia within your organization or immerse in phosphate buffer,respectively at 1,3,7,11,14 and 21 days out,gross morphology change of observation material,through histopathological observation material tissues around the presence of inflammation,accurate weighing to calculate presented.according to the weight of the materials,understanding of biodegradable polymer hemostatic materials in vivo and in vitro degradation of cellulose and histocompatibility.The rat liver bleeding wound model was prepared,and the local hemostasis was performed with this material and ordinary medical gauze respectively.The biodegradable hemostatic material of carboplatin and the extracts of blank cellulose material were injected into mice,and the acute systemic toxicity experiment was conducted to observe the general reaction and the changes of serological indexes.Results: The biodegradable hemostatic material containing carboplatin was basically degraded in vitro and in vivo for about 2 weeks,and was completely degraded within 3 weeks.No severe adverse reactions such as abscess formation and tissue necrosis were found in the surrounding tissues of the buried material.Inflammatory cell infiltration was observed in the early stage of implantation,and gradually recovered after 3 days.In terms of hemostasis effect on liver wounds,the hemostasis time and blood loss in the carboplatin biodegradable hemostatic material group were better than that in the ordinary gauze group and the blank control group(P < 0.05).Acute systemic toxicity test found that all mice in the experimental group and the control group survived,and no toxic reaction or other abnormalities were found.There were no statistically significant differences among the groups of serum indicators,such as white blood cells,liver transaminase and urea nitrogen(P >0.05).Conclusion: The new biodegradable carboplatin hemostatic material developed in this study has obvious hemostatic effect,good histocompatibility,suitable biodegradation time,no obvious toxic and side effects,and has potential clinical application value. |
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