Relaxant Action Of Coptisine On Mouse Airway Smooth Muscle

As research on lung disease continues to deepen,research on asthma is increasingly focused on safer and milder Chinese medicine.China's vast land and natural resources,has a very rich herbal medicine,and has found a variety of natural herbal extracts or a single compound to relax the pre-contracted airway muscles.Recently,our research has focused on the screening of traditional Chinese medicines for abnormal airway smooth muscle contraction.Coptisine is an active ingredient extracted from bitter herbs and has a range of functions,such as antibacterial,anti-oxidant,protective nerves and protects the heart muscle.In the current study,we questioned whether Coptisine can also play a role in asthma treatment by relaxing the abnormally contracted mouse tracheal rings.The aim of this study was to investigate the effects of abnormal smooth muscle relaxation and explore its underlying mechanisms.METHODS:The effects of Coptisine on mouse airway smooth muscle?ASM?cells were studied by a range of biophysiology techniques,including tension measurement and patch clamp experiments,ect.RESULTS:Coptisine dose-dependently relax hypertonic K⁺or acetylcholine?ACh?-induced pre-contracted mouse tracheal rings.Further studies have shown that the relaxation of Coptisine on tracheal smooth muscle is achieved by blocking voltage-dependent L-type Ca²⁺channels?VDLCCs?and non-selective cation channels?NSCCs?.The experimental results show that Coptisine achieves relaxation of pre-contracted tracheal smooth muscle by inhibiting the influx and release of calcium ions by inhibiting the VDCCs channel and the NSCCs channel.This also suggests that Coptisine may be used to treat diseases caused by abnormal contraction of tracheal smooth muscle.Therefore,Coptisine may have potential therapeutic value in the treatment of asthma and lung disease associated with abnormal airway smooth muscle contraction.