AID Modulates Carcinogenesis Network Via DNA Demethylation In Bladder Urothelium Cell Carcinoma

Objective To investigate the expression of AID(Activation-induced cytosine deaminase)in human bladder cancer and para-cancerous tissues and the relationship between AID and bladder cancer.;clear and definite the effect and mechanism of AID on proliferation,invasion,migration and apoptosis of bladder cancer cell line T24 and 5637;explor the effect of AID on the proliferation and metastasis of bladder cancer cells in nude mice Methods The relative expression of AID in 16 cases of bladder cancer tissues(radical cystectomy)was measured by Western Bolt and immunehistochemical staining;lentivirus-based sh RNA sequence was used to down-regulate the expression of AID in bladder cancer cell line T24 and 5637,nonsense sequence was used as negative control.Western Bolt was used to detected the rate of inhibition.The screening of monoclonal cells was performed,and cells with the lowest expression of AID was selected by Western Bolt for following research;To explor the effect of AID on T24 cells through Transwell assay,Cell Counting Kit 8(CCK8)and Flow cytometry;Observed the proliferation and metastasis of AID-silenced bladder cancer cells in nude mice;i TRAQ-based proteomic analysis was used to identify proteins with significantly different expression,bioinformatics approch was used to screened out the target factors and confirmed by Western Bolt the expression of selected protein was measured by Western Bolt;and 5aza-2'deoxycytidine was used to reveal that AID promote the EMT of T24 and 5637 through MMP14.Result Ectopic expression of AID was found in bladder cancer tissues,and relative expression level is significantly higher than that in paracancerous tissues(*P<0.05).Cell experiments confirmed that the inhibition of AID expression by RANi could significantly reduce the proliferation,migration and invasion ability of T24 cells(*P<0.05).Conclusion The expression of AID can affect the proliferation,migration and invasion of bladder cancer cells in vivo and vitro,and also increased the apoptosis in vitro.and its expression may be related to the occurrence and metastasis of bladder cancer.