Research On The Sensing Detection For Carcinoembryonic Antigen

Canceris is one of the major diseases threatening human life around the world,with prevention,screening and treatment of cancer,our country and the world will faces the major challenge of major diseases.Therefore,it is especially important to design and construct a rapid,accurate and simple detection method for cancer markers.This thesis constructs magnetic resonance biosensors and electrochemical biosensors with excellent properties of biosensors and nanomaterials for detecting carcinoembryonic antigens,the purpose of the study is to analyze different sensitive probes for the effect of carcinoembryonic antigens detection,which provide a new method for clinical application.The specific research contents of this thesis are as follows:First,based on the antibody immunoassay and resonance characteristics of magnetostrictive materials,a magnetoelastic(ME)Absensor was designed.With the specific binding principle of antibody-antigen,CEA antibody,a sensitive unit,was immobilized on the surface of the ME sensor.Bovine serum albumin(BSA)not only avoids non-specific adsorption,but also reduces steric hindrance between antibody and antibody.Atomic force microscopy(AFM)and scanningelectron microscopy(SEM)were used to characterize the microstructure of ME sensor,the FITC-labeled carcinoembryonic antigen antibody(FITC/Anti-CEA)was characterized by fluorescence microscopy to complete optimization of concentration for antibody,the prepared biosensor was used to detect the CEA.Then,based on the specifical immunoassay of aptamer,a novel ME aptasensor was prepared,thiolated aptamer,a sensitive unit,was self-assembled on the surface of the ME sensor.6-Mercapto-1-Hexanol(MCH)not only reduces the non-specific adsorption but also avoids steric effects of the aptamers.The surface morphology of the sensor was characterized by AFM and SEM,the elements of ME sensor were analyzed by EDS.The experimental conditions were optimized,ME aptasensor was used to quantitatively analyze CEA.Finally,gold nanoparticles(Au NPs)were prepared by chloroauric acid,which was reduced with sodium citrate and tannic acid,the compostie materials of Au NPs/MWCNTs were prepared with carboxylated Multi-Walled carbon nanotubes and gold nanoparticles.The morphology and electrical properties of MWCNTs and Au NPs/MWCNTs were characterized by SEM,EDS and CV.At the same time,the nanomaterials and the concentration of the aptamer were optimized.Under the optimal experimental conditions,the prepared electrochemical immunosensor was used to quantitatively detect CEA.